Immunofluorescence (IF)
(Figure 1. Indirect immunofluorescence staining of normal human dermal fibroblasts in tissue culture with (diluted 1:250), showing the specific cytoskeletal pattern of vimentin intermediate filaments.)
vimentin Recombinant Antibody
Recombinant mouse anti vimentin
Immunofluorescence (IF)
(Figure 1. Indirect immunofluorescence staining of normal human dermal fibroblasts in tissue culture with (diluted 1:250), showing the specific cytoskeletal pattern of vimentin intermediate filaments.)
Immunofluorescence (IF)
(Figure 1. Indirect immunofluorescence staining of normal human dermal fibroblasts in tissue culture with (diluted 1:250), showing the specific cytoskeletal pattern of vimentin intermediate filaments.)
Immunoflourescence (IF)
(Figure 2. Indirect immunofluorescence staining of human kidney tissue section with (diluted 1:1000), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, podocytes, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment)
Immunoflourescence (IF)
(Figure 2. Indirect immunofluorescence staining of human kidney tissue section with (diluted 1:1000), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, podocytes, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment)
Immunoflourescence (IF)
(Figure 3. Indirect immunofluorescence staining of human kidney tissue section with (diluted 1:1000), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, podocytes, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment)
Immunoflourescence (IF)
(Figure 3. Indirect immunofluorescence staining of human kidney tissue section with (diluted 1:1000), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, podocytes, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment)
Immunohistochemistry (IHC)
(Figure 4. Immunostaining of human paraffin embedded tissue section of placenta with (diluted 1:100), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment)
Immunohistochemistry (IHC)
(Figure 4. Immunostaining of human paraffin embedded tissue section of placenta with (diluted 1:100), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment)
Immunohistochemistry (IHC)
(Figure 5. Immunostaining of human paraffin embedded tissue section of testis with (diluted 1:100), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, endothelial cells in blood vessels and Sertoli cells.)
Immunohistochemistry (IHC)
(Figure 5. Immunostaining of human paraffin embedded tissue section of testis with (diluted 1:100), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, endothelial cells in blood vessels and Sertoli cells.)
Immunohistochemistry (IHC)
(Figure 6. Immunostaining of human paraffin embedded tissue sections of human colon with (diluted 1:100), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment.)
Immunohistochemistry (IHC)
(Figure 6. Immunostaining of human paraffin embedded tissue sections of human colon with (diluted 1:100), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment.)
Immunohistochemistry (IHC)
(Figure 7. Immunostaining of human paraffin embedded tissue sections of human kidney with (diluted 1:100), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, and podocytes. As expected, no reactivity is seen in the epithelial cell compartment.)
Immunohistochemistry (IHC)
(Figure 7. Immunostaining of human paraffin embedded tissue sections of human kidney with (diluted 1:100), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, and podocytes. As expected, no reactivity is seen in the epithelial cell compartment.)
Immunohistochemistry (IHC)
(Figure 8. Immunostaining of human paraffin embedded tissue sections of human small intestine with (diluted 1:100), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue. As expected, no reactivity is seen in the epithelial cell compartment.)
Immunohistochemistry (IHC)
(Figure 8. Immunostaining of human paraffin embedded tissue sections of human small intestine with (diluted 1:100), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue. As expected, no reactivity is seen in the epithelial cell compartment.)
Immunohistochemistry (IHC)
(Figure 9. Immunostaining of human paraffin embedded tissue sections of human spleen with (diluted 1:100), showing the specific pattern of vimentin in the mesenchymal cell types.)
Immunohistochemistry (IHC)
(Figure 9. Immunostaining of human paraffin embedded tissue sections of human spleen with (diluted 1:100), showing the specific pattern of vimentin in the mesenchymal cell types.)
Western Blot (WB)
(Figure 10. Western blotting result showing the specific reactivity of with the 57kDa protein band of vimentin in both the mouse (3T3 mouse fibroblasts; left lane) and human (normal human dermal fibroblasts; right lane) cell extracts.)
Western Blot (WB)
(Figure 10. Western blotting result showing the specific reactivity of with the 57kDa protein band of vimentin in both the mouse (3T3 mouse fibroblasts; left lane) and human (normal human dermal fibroblasts; right lane) cell extracts.)
SDS-Page
(Figure 11. Non-reduced and reduced SDS-PAGE of showing its purity.)
SDS-Page
(Figure 11. Non-reduced and reduced SDS-PAGE of showing its purity.)
Similar Products
Product Notes
The vimentin (Catalog #AAA574008) is a Recombinant Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Recombinant mouse anti vimentin reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's vimentin can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) Frozen/Paraffin, Immunoblot (IB). (clone RV204) is suitable for immunoblotting, immunocytochemistry, and immunohistochemistry on frozen and paraffin-embedd tissue sections. Optimal antibody dilution should be determined by titration; recommended range is 1:100 – 1:200 for immunohistochemistry with fluorochrome conjugated secondary antibodies or with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications. Researchers should empirically determine the suitability of the vimentin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "vimentin, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
