Rat MMP-2 ELISA Kit | MMP2 elisa kit

Rat MMP-2 PicoKine ELISA Kit

Cat. Num. AAA175878

• Reactivity: Rat
• Synonyms: MMP-2; Rat MMP-2 PicoKine ELISA Kit; 72 kDa type IV collagenase; matrix metallopeptidase 2 (gelatinase A; 72kDa gelatinase; 72kDa type IV collagenase); 72 kDa gelatinase; CLG 4; CLG 4A; CLG4; CLG4A; Collagenase type IV A; EC 3.4.24.24; Gelatinase A; Gelatinase alpha; Gelatinase neutrophil; Matrix metallopeptidase 2 (gelatinase A; Matrix metalloproteinase 2; Matrix metalloproteinase 2 (gelatinase A; 72kD gelatinase; 72kD type IV collagenase); Matrix metalloproteinase II; Matrix metalloproteinase-2; MMP 2; MMP II; MMP2; MMP2_HUMAN; MONA; Neutrophil gelatinase; PEX; TBE 1; TBE-1; MMP2 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant rat total MMP-2
• Sequence Length: 662

• Samples: Cell culture supernates, serum and plasma (heparin)
• Assay Type: Sandwich
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: < 10pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Rat MMP-2 PicoKine ELISA Kit standard curve )

Related Product Information for MMP2 elisa kit

Principle of the assay: rat MMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MMP-2 has been precoated onto 96-well plates. Standards (NSO,A30-C662) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat MMP-2 amount of sample captured in plate.
Background: Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2 (MMP2). It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown.1 Matrix metalloproteinases (MMPs) catalyze extracellular matrixdegradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide (cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability.2 The gene is localized to 16q21 using somatic cell hybrids and in situhybridization.3 The standard product used in this kit is recombinant rat MMP-2, consisting of 662 amino acids withthe molecular mass of 72KDa. The detected MMP-2 includes zymogen and active enzyme.

NCBI and Uniprot Product Information

• NCBI GI #: 146345459
• NCBI GeneID: 81686
• UniProt Accession #: P33436; P97581; Q6GMM9
• Molecular Weight: 74,149 Da
• NCBI Official Full Name: 72 kDa type IV collagenase
• NCBI Official Synonym Full Names: matrix metallopeptidase 2
• NCBI Official Symbol: Mmp2
• NCBI Protein Information: 72 kDa type IV collagenase; MMP-2; gelatinase A; 72 kDa gelatinase; matrix metalloproteinase 2; matrix metalloproteinase-2
• UniProt Protein Name: 72 kDa type IV collagenase
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: Mmp2
• UniProt Synonym Gene Names: MMP-2
• UniProt Entry Name: MMP2_RAT

NCBI Description

plays important roles in wound healing, angiogenesis, platelet aggregation, and tumor metastasis; mediates the response of the intrinsic glomerular mesangial cell to inflammatory stimuli [RGD, Feb 2006]

Uniprot Description

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues ().