Testing Data
Mouse Angiopoietin-2 ELISA Kit | Angpt2 elisa kit
Mouse Angiopoietin-2 Quick ELISA Kit
O.D.|0.030|0.153|0.321|0.669|1.026|1.628|2.072|2.434
n|16|16|16|
Mean(pg/ml)|218|1324|4652|
Standard deviation|11.12|55.61|250.91|
CV(%)|5.1%|4.2%|5.5%|
n|24|24|24
Mean(pg/ml)|226|1236|4966
Standard deviation|10.62|59.33|332.72
CV(%)|4.7%|4.8%|6.7%
Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%)
Sample 1: 218|210|235|266|232|10.92|4.7%
Sample 2: 1324|1236|1234|1241|1259|44.06|3.5%
Sample 3: 4562|5698|5204|5784|5312|350.59|6.6%
*number of samples for each test n=16.
This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
To measure Mouse Angpt2, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Angpt2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Angpt2 in the sample.
Testing Data
Testing Data
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an endothelial cell (EC)-derived regulator of angiogenesis and ligand for endothelial-specific receptor tyrosine kinase. The encoded protein acts as an anti-apoptotic factor for stressed ECs and a proapoptotic factor for resting ECs. [provided by RefSeq, Jan 2013]
Uniprot Description
ANGPT2: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. Interacts with TEK/TIE2, competing for the same binding site as ANGPT1. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 8 A1.3|8 10.3 cM
Cellular Component: cell projection; extracellular region; extracellular space; nucleus; plasma membrane
Molecular Function: metal ion binding; protein binding; receptor tyrosine kinase binding; vascular endothelial growth factor receptor binding
Biological Process: angiogenesis; blood vessel morphogenesis; blood vessel remodeling; cell differentiation; endoderm development; hemopoiesis; multicellular organism development; negative regulation of angiogenesis; negative regulation of blood vessel endothelial cell migration; negative regulation of positive chemotaxis; positive regulation of angiogenesis; regulation of angiogenesis; Tie signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway
Research Articles on Angpt2
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Product Notes
The Mouse Angpt2 angpt2 (Catalog #AAA1753941) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1753941 ELISA Kit recognizes Mouse Angpt2. It is sometimes possible for the material contained within the vial of "Angiopoietin-2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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