Rat Rantes ELISA Kit | Ccl5 elisa kit

Rat Rantes Quick ELISA Kit

Cat. Num. AAA1753920

• Gene Names: Ccl5; Scya5; Rantes
• Reactivity: Rat
• Synonyms: Rantes; Rat Rantes Quick ELISA Kit; CCL5/RANTES|CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed; and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys); member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES; Ccl5 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant Rat Ccl5

• Assay Type: Colorimetric sandwich
• Samples: cell culture supernatants. serum and plasma (heparin, EDTA, citrate)
• Detection Range: 15.6pg/ml-1,000pg/ml
• Sensitivity: <1pg/ml
• Capture/Detection Antibodies: Capture antibody- polyclonal antibody from goat|Detection antibody- polyclonal antibody from goat
• Sample Data: Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000; O.D.|0.016|0.164|0.255|0.450|0.831|1.445|2.087|2.262
• Intra-Assay Precision: Sample|1|2|3|; n|16|16|16|; Mean(pg/ml)|32|212|364|; Standard deviation|2.02|16.32|20.02|; CV(%)|6.3%|7.7%|5.5%|
• Inter-Assay Precision: Sample|1|2|3|; n|24|24|24; Mean(pg/ml)|36|199|351; Standard deviation|2.74|14.13|25.62; CV(%)|7.6%|7.1%|7.3%
• Reproducibility: To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.; Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%); Sample 1: 32|30|36|29|32|1.65|5.2%; Sample 2: 212|180|196|206|199|9.73|4.9%; Sample 3: 364|397|405|374|385|6.93|1.8%; *number of samples for each test n=16.
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: E Coli; Immunogen sequence: S24-S91
• Anticoagulant: heparin, EDTA or citrate
• Benefits: The Quick ELISA kits, assay takes less than 1.5 hours. Detect Rat Rantes with <1pg/ml sensitivity. Format: 96-well plate with removable strips.; This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
• Principle: Rat Ccl5 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Ccl5 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit contains recombinant Rat Ccl5 with immunogen: Expression system for standard: E Coli; Immunogen sequence: S24-S91.; ; To measure Rat Ccl5, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Rat Ccl5 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Ccl5 in the sample.
• Assay Procedure Step 1: 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml
• TMB Incubation Time: 15
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)

Testing Data

Related Product Information for Ccl5 elisa kit

Rantes(regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4(+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.

Product Categories/Family for Ccl5 elisa kit

ELISA kits

NCBI and Uniprot Product Information

• NCBI GI #: 1711393
• NCBI GeneID: 81780
• UniProt Accession #: P50231
• Molecular Weight: 10,170 Da
• NCBI Official Full Name: C-C motif chemokine 5
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 5
• NCBI Official Symbol: Ccl5
• NCBI Official Synonym Symbols: Scya5; Rantes
• NCBI Protein Information: C-C motif chemokine 5; SIS-delta; T-cell-specific protein RANTES; regulated upon activation normal T-cell expressed and secreted; small inducible cytokine A5; small-inducible cytokine A5
• UniProt Protein Name: C-C motif chemokine 5
• Protein Family: C-C motif chemokine
• UniProt Gene Name: Ccl5
• UniProt Synonym Gene Names: Scya5
• UniProt Entry Name: CCL5_RAT

NCBI Description

may play a role in cellular response to viral infection in testicular somatic cells [RGD, Feb 2006]

Uniprot Description

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.