Mouse KIM1/TIM1 ELISA Kit | Havcr1 elisa kit

Mouse KIM1/TIM1 Quick ELISA Kit

Cat. Num. AAA1753938

• Gene Names: Havcr1; Tim1; KIM-1; TIM-1; Timd1; AI503787
• Reactivity: Mouse
• Synonyms: KIM1/TIM1; Mouse KIM1/TIM1 Quick ELISA Kit; TIM-1/KIM-1/HAVCR|CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1; Havcr1 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Havcr1

• Assay Type: Colorimetric sandwich
• Samples: cell culture supernatants, serum, plasma (heparin, EDTA) and urine
• Detection Range: 31.2pg/ml-2,000pg/ml
• Sensitivity: <2pg/ml
• Capture/Detection Antibodies: Capture antibody- polyclonal antibody from goat|Detection antibody- polyclonal antibody from goat
• Sample Data: Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000; O.D.|0.049|0.144|0.221|0.371|0.656|1.143|1.803|2.355
• Intra-Assay Precision: Sample|1|2|3|; n|16|16|16|; Mean(pg/ml)|83|296|863|; Standard deviation|6.47|16.28|36.25|; CV(%)|7.8%|5.5%|4.2%|
• Inter-Assay Precision: Sample|1|2|3|; n|24|24|24; Mean(pg/ml)|79|265|805; Standard deviation6.95|14.05|37.84; CV(%)|8.8%|5.3%|4.7%
• Reproducibility: To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.; Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%); Sample 1: 83|91|91|99|91|3.19|3.5%; Sample 2: 296|269|241|277|271|13.81|5.1%; Sample 3: 863|722|826|797|802|42.51|5.3%; *number of samples for each test n=16.
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: Y22-T212
• Anticoagulant: heparin or EDTA
• Benefits: The Quick ELISA kits, assay takes less than 1.5 hours. Detect Mouse KIM1/TIM1 with <2pg/ml sensitivity. Format: 96-well plate with removable strips.; This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
• Principle: Mouse Havcr1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Havcr1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit contains recombinant Mouse Havcr1 with immunogen: Expression system for standard: NS0; Immunogen sequence: Y22-T212.; ; To measure Mouse Havcr1, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Havcr1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Havcr1 in the sample.
• Assay Procedure Step 1: 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.25pg/ml
• TMB Incubation Time: 15
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)

Testing Data

Related Product Information for Havcr1 elisa kit

KIM1(TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Mouses is encoded by the HAVCR1 gene. Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led Feigelstock et al. (1998) to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to mouse Tim1, Umetsu et al. (2005) showed that Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the mouse Tim1 gene and database analysis, McIntire et al. (2001) mapped the HAVCR1 gene to 5q33. 2. The standard used in this kit is recombinant mouse KIM-1, constituting from Y22-T212 amino acids, and 191amino acids with the molecular mass of 21. 8KDa.

Product Categories/Family for Havcr1 elisa kit

ELISA kits

NCBI and Uniprot Product Information

• NCBI GI #: 262231823
• NCBI GeneID: 171283
• NCBI Accession #: NP_001160103.1
• NCBI GenBank Nucleotide #: NM_001166631.1
• UniProt Accession #: Q5QNS5; Q7TPU2; Q8VIM1; Q8VIM2
• Molecular Weight: 30,925 Da
• NCBI Official Full Name: hepatitis A virus cellular receptor 1 homolog isoform b
• NCBI Official Synonym Full Names: hepatitis A virus cellular receptor 1
• NCBI Official Symbol: Havcr1
• NCBI Official Synonym Symbols: Tim1; KIM-1; TIM-1; Timd1; AI503787
• NCBI Protein Information: hepatitis A virus cellular receptor 1 homolog
• UniProt Protein Name: Hepatitis A virus cellular receptor 1 homolog
• Protein Family: Hepatitis A virus cellular receptor
• UniProt Gene Name: Havcr1
• UniProt Synonym Gene Names: Kim1; Tim1; Timd1; HAVcr-1; KIM-1; TIMD-1; TIM-1
• UniProt Entry Name: HAVR1_MOUSE

Uniprot Description

TIM-1: May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4. In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair. Belongs to the immunoglobulin superfamily. TIM family.

Protein type: Membrane protein, integral

Cellular Component: cell surface

Molecular Function: phosphatidylserine binding; protein binding

Biological Process: positive regulation of mast cell activation