Testing Data
Rat EGF ELISA Kit | Egf elisa kit
Rat EGF Quick ELISA Kit
O.D.|0.082|0.173|0.222|0.373|0.592|1.032|1.721|2.378
n|16|16|16|
Mean(pg/ml)|12|88|241|
Standard deviation|0.79|4.66|16.87|
CV(%)|6.6%|5.3%|7%|
n|24|24|24
Mean(pg/ml)|9|91|224
Standard deviation|0.73|6.28|17.47
CV(%)|8.1%|6.9%|7.8%
Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%)
Sample 1: 12|11|10|11|11|0.36|3.3%
Sample 2: 88|90|86|93|89|5.80|6.5%
Sample 3: 241|212|229|243|231|8.79|3.8%
*number of samples for each test n=16.
This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
To measure Rat Egf, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Rat Egf in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Egf in the sample.
Testing Data
Testing Data
NCBI and Uniprot Product Information
NCBI Description
binds the epidermal growth factor receptor; may play a role in MAP kinase mediated signaling pathways [RGD, Feb 2006]
Uniprot Description
EGF: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4); also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ligand, receptor tyrosine kinase; Hormone; Membrane protein, integral
Cellular Component: extracellular space; lysosomal membrane; plasma membrane; integral to membrane; intracellular
Molecular Function: growth factor activity; calcium ion binding; epidermal growth factor receptor binding
Biological Process: epidermal growth factor receptor signaling pathway; peptidyl-tyrosine phosphorylation; activation of MAPKK activity; positive regulation of mitosis; positive regulation of transcription, DNA-dependent; regulation of protein transport; positive regulation of MAP kinase activity; regulation of protein secretion; branching morphogenesis of a tube; positive regulation of epidermal growth factor receptor activity; positive regulation of cell proliferation; negative regulation of secretion; angiogenesis; regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphorylation; STAT protein nuclear translocation; positive regulation of DNA binding; positive regulation of granule cell precursor proliferation
Research Articles on Egf
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Product Notes
The Rat Egf egf (Catalog #AAA1753942) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1753942 ELISA Kit recognizes Rat Egf. It is sometimes possible for the material contained within the vial of "EGF, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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