Testing Data
Mouse Lipocalin-2/NGAL ELISA Kit | Lcn2 elisa kit
Mouse Lipocalin-2/NGAL Quick ELISA Kit
O.D.|0.014|0.131|0.237|0.425|0.807|1.353|2.161|2.347
n|16|16|16|
Mean(pg/ml)|139|827|2674|
Standard deviation|7.5|48.79|117.65|
CV(%)|5.4%|5.9%|4.4%|
n|24|24|24
Mean(pg/ml)|144|858|2755
Standard deviation|8.35|57.48|143.26
CV(%)|5.8%|6.7%|5.2%
Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%)
Sample 1: 139|135|141|147|140|5.46|3.9%
Sample 2: 827|767|772|849|803|35.33|4.4%
Sample 3: 2674|2773|2312|2421|2545|173.06|6.8%
*number of samples for each test n=16.
This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
To measure Mouse Lcn2, add standards and samples to the wells, then add HRP linked detection antibody. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Lcn2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Lcn2 in the sample.
Testing Data
Testing Data
NCBI and Uniprot Product Information
Uniprot Description
LCN2: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. Belongs to the calycin superfamily. Lipocalin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: cytosol; extracellular region; extracellular space
Molecular Function: iron ion binding; protease binding; protein binding; protein homodimerization activity
Biological Process: innate immune response; positive regulation of cell projection organization and biogenesis; response to drug; response to herbicide; response to virus; siderophore transport
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Product Notes
The Mouse Lcn2 lcn2 (Catalog #AAA1753935) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1753935 ELISA Kit recognizes Mouse Lcn2. It is sometimes possible for the material contained within the vial of "Lipocalin-2/NGAL, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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