Mammalian Threonine Assay Kit

PicoProbe Threonine Assay Kit (Fluorometric)

Cat. Num. AAA849685

• Reactivity: Mammalian
• Synonyms: Threonine; PicoProbe Threonine Assay Kit (Fluorometric); Threonine assay kit

• Reactivity: Mammalian

• Samples: Human or animal biological fluids (plasma, serum, CSF, etc.); Soft tissuehomogenates (i.e. liver, brain, etc.); Cultured cell lysates (adherent or suspension cells)or cell culture growth medium
• Detection Method: Fluorescence: Ex/Em 535/587nm
• Features & Benefits: Market's first and only kit for enzymatic detection of Threonine in biological samples; Simple, rapid & convenient assay; Limit of detection: 2 uM; Very specific, does not interact with other standard and non-standard amino acids
• Preparation and Storage: Store at -20 degree C; Shelf life: ~12 months

Testing Data

Testing Data

Testing Data

Related Product Information for Threonine assay kit

Threonine is one of the 20 naturally-occuring amino acids used by all organisms in the biosynthesis of proteins and is an essential dietary amino acid for humans. Threonine residues in proteins are subject to post-translational phosphorylation, a critical regulatory and signal transduction mechanism. In addition to its role as a protein building block, threonine can serve as a precursor to other amino acids and metabolites, including glycine, isoleucine and acetyl-coenzyme A. Recent research has shown that threonine is a vital nutrient for embryonic stem cells and threonine metabolism (mediated by the enzyme threonine dehydrogenase) is required for unlimited self-renewal and maintenance of stem cell pluripotency. Dietary threonine deficiency, while rare, can result in breakdown of the intestinal mucosal lining (eventually triggering inflammatory bowel disorders), immunosuppression and a multitude of neuropsychiatric symptoms. Threonine Assay Kit allows for highly sensitive quantification of L-Threonine levels in biological fluids and tissues. The assay is based on the selective, NAD+-coupled enzymatic metabolism of threonine, yielding an oxidized intermediate and NADH. A developer enzyme mixture utilizes the NADH generated to convert PicoProbe into a stable fluorophore (Ex/Em = 538/587 nm). The assay is not affected by physiological concentrations of other amino acids, is high-throughput adaptable and can detect threonine levels down to 2 uM in samples

Product Categories/Family for Threonine assay kit

Amino Acid Metabolism; Metabolism Assays; Amino Acids & Proteins; Metabolism Assays (A-Z)