Testing Data
Eukaryotes Phosphatidylserine Assay Kit
Phosphatidylserine Assay Kit (Fluorometric)
Reactivity
Eukaryotes
Synonyms
Phosphatidylserine; Phosphatidylserine Assay Kit (Fluorometric); Phosphatidylserine assay kit
Reactivity
Eukaryotes
Samples
Soft tissue homogenates (i.e. liver, brain, etc.)
Biological fluids (serum/plasma)
Cultured cell lysates (adherent or suspension cells)
Biological fluids (serum/plasma)
Cultured cell lysates (adherent or suspension cells)
Detection Method
Fluorescence: Ex/Em 535/587nm
Features & Benefits
Simple, fast, does not require lenghty incubation times
The first kit in the market to measure Phosphatidylserine in samples
This assay provides a convenient and non-ELISA alternative to detect Phosphatidylserine amount in biological samples.
The first kit in the market to measure Phosphatidylserine in samples
This assay provides a convenient and non-ELISA alternative to detect Phosphatidylserine amount in biological samples.
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Testing Data
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Related Product Information for Phosphatidylserine assay kit
Phosphatidylserine (PS) is a glycerophospholipid consisting of a phosphatidyl group attached to L-serine via a phosphodiester linkage. PS is a critical component of the cellular plasma membrane and accounts for 2-15% of plasma membrane lipid composition, depending on the cell/tissue type. The highest concentrations of PS are found in neuronal tissues, where it is critical for maintaining conduction velocity in myelinated neurons, as well as for higher order cognitive skills such as learning and memory. In normal, healthy cells, PS is held in the inner membrane surface (facing the cytosol) by the lipid transporter protein flippase. However, in apoptotic cells, PS molecules 'shuffle' between the inner and outer plasma membrane monolayers. When PS molecules flip to the extracellular (outer) surface of the cell membrane, they act as a signal for macrophages to engulf and digest the (apoptotic) cell. Phosphatidylserine Assay Kit allows for quantification of PS in lipid extracts of cell/tissue lysates. The assay is based on the enzymatic cleavage of PS to yield phosphatidic acid and L-serine, which is subsequently metabolized and reacts with a probe to form a stable fluorophore (Ex/Em = 538/587 nm). The assay is selective for PS (other phospholipids such as phosphatidylcholine, phosphatidylethanolamine or phosphatidic acid do not interfere). The kit is high-throughput ready and highly sensitive (can detect as little as 50 pmole/well of PS (5 uM in a 10 ul sample volume) ) .
Product Categories/Family for Phosphatidylserine assay kit
Similar Products
Product Notes
The Eukaryotes Phosphatidylserine (Catalog #AAA849736) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849736 Assay Kit recognizes Eukaryotes Phosphatidylserine. It is sometimes possible for the material contained within the vial of "Phosphatidylserine, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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