Mammalian DL-Serine Assay Kit

DL-Serine Assay Kit (Fluorometric)

Cat. Num. AAA849756

• Reactivity: Mammalian
• Synonyms: DL-Serine; DL-Serine Assay Kit (Fluorometric); DL-Serine assay kit

• Reactivity: Mammalian

• Samples: Human or animal biological fluids (plasma, serum, CSF, etc.); Soft tissue homogenates (i.e. liver, brain, etc.); Cultured cell lysates (adherent or suspension cells)
• Detection Method: Fluorescence: Ex/Em 535/587nm
• Features & Benefits: Market's first and only kit for enzymatic detection of Serine in biological samples; Simple, rapid & convenient assay; Limit of detection: 1 uM; Very specific, does not interact with other standard and non-standard amino acids
• Preparation and Storage: Store at -20 degree C; Shelf life: ~12 months

Testing Data

Testing Data

Testing Data

Related Product Information for DL-Serine assay kit

Serine is one of the 20 naturally-occurring amino acids used by all organisms in the biosynthesis of proteins. Having a single chiral center, serine can exist as either of two stereoisomers (L-Serine and D-Serine). In addition to its role as a protein building block, L-Serine serves as a precursor to many vital biomolecules, including phosphatidylserine, sphingomyelin and the amino acids glycine and cysteine. The non-proteogenic isomer D-Serine is also synthesized from L-Serine in the mammalian brain. While most D-amino acids are only present in trace amounts in mammals, D-Serine is a vital neurotransmitter that acts as a co-agonist of synaptic N-methyl-D-aspartate (NMDA) type glutamate-gated ion channels. Impaired D-Serine metabolism may contribute to several neuropsychiatric disorders, including depression, schizophrenia, epilepsy and dementia. DL-Serine Assay Kit allows for quantification of both L- and D-Serine in biological fluids and tissues. The assay is based on the conversion of L-Serine to D-Serine, which is metabolized to an intermediate product that is subsequently oxidized and reacts with a probe to form a stable fluorophore (Ex/Em = 539/587 nm). Samples may be divided and assayed simultaneously for quantification of both D-Serine and total DL-Serine. The assay is not affected by physiological concentrations of other amino acids, is high-throughput adaptable and can detect less than 1 uM Serine

Product Categories/Family for DL-Serine assay kit

Amino Acid Metabolism; Metabolism Assays; Amino Acids & Proteins; Metabolism Assays (A-Z)