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Testing Data
Mammalian Leucine Aminopeptidase (LAP) Assay Kit
Leucine Aminopeptidase (LAP) Activity Assay Kit (Fluorometric)
Reactivity
Mammalian
Synonyms
Leucine Aminopeptidase (LAP); Leucine Aminopeptidase (LAP) Activity Assay Kit (Fluorometric); Leucine Aminopeptidase (LAP) assay kit
Reactivity
Mammalian
Samples
Animal tissues: liver, spleen, placenta, etc
Cell culture: Adherent or suspension cells
Cell culture: Adherent or suspension cells
Detection Method
Fluorometric: Ex/Em 368/460nm
Features & Benefits
Highly Sensitive, minimum sample preparation required
Simple one-step reaction
Takes only 1-2 hrs
Non-radiometric fluorescent detection
HTP adaptable
Detection of LAP activity in mammalian tissues, cell culture and purified enzyme
Simple one-step reaction
Takes only 1-2 hrs
Non-radiometric fluorescent detection
HTP adaptable
Detection of LAP activity in mammalian tissues, cell culture and purified enzyme
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
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Related Product Information for Leucine Aminopeptidase (LAP) assay kit
Leucine aminopeptidases (EC 3.4.11.1) (LAPs) are a diverse set of exopeptidases that catalyze the hydrolysis of leucine residues from the amino-termini of proteins or peptides. LAPs are ubiquitous enzymes present among animals, plants and prokaryotes. Previously, they were thought to typically play important roles in cell maintenance, growth and development. However, research in the recent years has identified multiple secondary functions for these enzymes in animals and microbes including transcriptional regulation and vesicle transport. Studies have implicated LAP enzymes in tumor cell proliferation, invasion and angiogenesis. Placental LAP is used as a biomarker in ovarian epithelial cancer while adipocyte-derived LAP is used as a marker of endometrial cancer cell proliferation and differentiation. LAP enzymes are also known to be involved in catabolism of oxytocin and vasopressin and insulin regulation of GLUT4 receptors in diabetes. Leucine Aminopeptidase assay kit provides a quick, sensitive and easy way for measuring total LAP activity in various samples. In this assay, LAPs hydrolyze leucine from the fluorescent probe and the amount of fluorescent probe detected at Ex/Em 368/460 nm is used to determine the total activity of the LAP enzymes. The assay is simple to perform, high-throughput adaptable and can detect less than 0.1 mU of LAP activity
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Product Notes
The Mammalian Leucine Aminopeptidase (LAP) (Catalog #AAA849720) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849720 Assay Kit recognizes Mammalian Leucine Aminopeptidase (LAP). It is sometimes possible for the material contained within the vial of "Leucine Aminopeptidase (LAP), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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