Testing Data
Mammalian Aminopeptidase N (APN/CD13) Assay Kit
Aminopeptidase N (APN/CD13) Activity Assay Kit (Fluorometric)
Reactivity
Mammalian
Synonyms
Aminopeptidase N (APN/CD13); Aminopeptidase N (APN/CD13) Activity Assay Kit (Fluorometric); Aminopeptidase N (APN/CD13) assay kit
Reactivity
Mammalian
Samples
Lysates of soft tissues (e.g.kidney, liver, lung) or cultured cells
Human or animal biological fluids (e.g.serum, plasma)
Human or animal biological fluids (e.g.serum, plasma)
Detection Method
Fluorometric: Ex/Em 384/502nm
Features & Benefits
Highly Sensitive, minimum sample preparation required
Simple one-step reaction
Takes only 1-2 hrs
Non-radiometric fluorescent detection
HTP adaptable
Detection of CD13 activity in mammalian tissues, cell culture and purified enzyme
Simple one-step reaction
Takes only 1-2 hrs
Non-radiometric fluorescent detection
HTP adaptable
Detection of CD13 activity in mammalian tissues, cell culture and purified enzyme
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Testing Data
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Related Product Information for Aminopeptidase N (APN/CD13) assay kit
Aminopeptidase N (APN, EC 3.4.11.2) also known as CD13 or membrane alanyl aminopeptidase is a Zn2+-dependent ectopeptidase that cleaves N-terminal neutral amino acids (preferentially alanine) from proteins and peptides. APN is a promiscuous, multifunction enzyme consisting of a short cytoplasmic domain, a single transmembrane helix and a large extracellular catalytic domain that can be cleaved to generate a soluble version of the enzyme. Aminopeptidase N expression is upregulated in many different human cancers and the enzyme is involved in metastatic tumor cell proliferation, invasion and angiogenesis. Serum levels of soluble APN are elevated in cancer patientscompared with healthy controls and there is a strong inverse correlation between serum APN, tumor load and long-term prognosis in pancreatic, breast and colon cancers. Thus, in addition to being a promising pharmacological target for novel anti-neoplastic drugs, serum APN activity has been proposed as a non-invasive biomarker for the test and surveillance of breast and pancreatic cancers. Aminopeptidase N Activity Assay Kit enables rapid measurement of APN activity, utilizing a fluorogenic substrate that is converted into a highly fluorescent product (Ex/Em = 384/502 nm). A selective APN inhibitor is provided for verification of specific activity. The assay is simple, high-throughput adaptable and can detect a minimum of 100 uU APN activity in a variety of biological samples
Product Categories/Family for Aminopeptidase N (APN/CD13) assay kit
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Product Notes
The Mammalian Aminopeptidase N (APN/CD13) (Catalog #AAA849714) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849714 Assay Kit recognizes Mammalian Aminopeptidase N (APN/CD13). It is sometimes possible for the material contained within the vial of "Aminopeptidase N (APN/CD13), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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