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Western Blot (WB) (LASS1 monoclonal antibody (M04), clone 3F9. Western Blot analysis of LASS1 expression in HepG2.)

Mouse LASS1 Monoclonal Antibody | anti-LASS1 antibody

LASS1 (LAG1 Homolog, Ceramide Synthase 1, CerS1, LAG1, MGC90349, UOG1) (Biotin)

Gene Names
CERS1; LAG1; UOG1; LASS1
Applications
ELISA, Western Blot
Purity
Purified
Synonyms
LASS1; Monoclonal Antibody; LASS1 (LAG1 Homolog; Ceramide Synthase 1; CerS1; LAG1; MGC90349; UOG1) (Biotin); LAG1 Homolog; UOG1; anti-LASS1 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
3F9
Specificity
Recognizes LASS1.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-LASS1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
LASS1 (NP_067090.1, 301aa-350aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
YIVAFAAKVLTGQVHELKDLREYDTAEAQSLKPSKAEKPLRNGLVKDKRF
Conjugate
Biotin
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(LASS1 monoclonal antibody (M04), clone 3F9. Western Blot analysis of LASS1 expression in HepG2.)

Western Blot (WB) (LASS1 monoclonal antibody (M04), clone 3F9. Western Blot analysis of LASS1 expression in HepG2.)

Testing Data

(Detection limit for recombinant GST tagged LASS1 is 0.03 ng/ml as a capture antibody.)

Testing Data (Detection limit for recombinant GST tagged LASS1 is 0.03 ng/ml as a capture antibody.)
Related Product Information for anti-LASS1 antibody
This gene encodes a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site that is cleaved to produce a mature protein containing seven conserved cysteine residues. Members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in yeast suggest that the encoded protein is involved in aging. This protein is transcribed from a monocistronic mRNA as well as a bicistronic mRNA, which also encodes growth differentiation factor 1. [provided by RefSeq]
Product Categories/Family for anti-LASS1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
39536
NCBI Official Full Name
ceramide synthase 1 isoform 1
NCBI Official Synonym Full Names
ceramide synthase 1
NCBI Official Symbol
CERS1
NCBI Official Synonym Symbols
LAG1; UOG1; LASS1
NCBI Protein Information
ceramide synthase 1; protein UOG-1; upstream of GDF1; longevity assurance gene 1 protein homolog 1; longevity assurance (LAG1, S. cerevisiae) homolog 1
UniProt Protein Name
Ceramide synthase 1
UniProt Gene Name
CERS1
UniProt Synonym Gene Names
LAG1; LASS1; UOG1; CerS1
UniProt Entry Name
CERS1_HUMAN

NCBI Description

This gene encodes a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site that is cleaved to produce a mature protein containing seven conserved cysteine residues. Members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in yeast suggest that the encoded protein is involved in aging. This protein is transcribed from a monocistronic mRNA as well as a bicistronic mRNA, which also encodes growth differentiation factor 1. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: May be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When overexpressed in cells is involved in the production of sphingolipids containing mainly one fatty acid donor (N-linked stearoyl- (C18) ceramide) in a fumonisin B1-independent manner

By similarity.

Subcellular location: Endoplasmic reticulum membrane; Multi-pass membrane protein

By similarity. Isoform 1: Endoplasmic reticulum membrane; Multi-pass membrane protein

By similarity. Golgi apparatus membrane; Multi-pass membrane protein

By similarity. Note: Isoform 1 may recycle from the Golgi to the endoplasmic reticulum.

Miscellaneous: This protein is produced by a bicistronic gene which also produces the GDF1 protein from a non-overlapping reading frame.

Sequence similarities: Contains 1 TLC (TRAM/LAG1/CLN8) domain.

Research Articles on LASS1

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Product Notes

The LASS1 cers1 (Catalog #AAA6174711) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's LASS1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the LASS1 cers1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LASS1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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