PRKAR1A recombinant protein

PRKAR1A Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8123223

• Gene Names: Prkar1a; Tse1; Tse-1; RIalpha; 1300018C22Rik
• Purity: >90% as determined by SDS-PAGE
• Synonyms: PRKAR1A; PRKAR1A Protein; Mouse; Recombinant (His Tag); Mouse PRKAR1A/PRKAR1/PKR1 Protein (His Tag); 1300018C22Rik Protein; RIalpha Protein; Tse-1 Protein; Tse1 Protein; protein kinase; cAMP-dependent; regulatory; type I; alpha; PRKAR1A recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 300mM NaCl, pH7.5, 10% glycerol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Val381

• Species: Mouse
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Met 1
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse PRKAR1A (NP_068680.1)(Met1-Val381) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PRKAR1A recombinant protein

Background: PRKAR1A, also known as PRKAR1 and PKR1, is one of the regulatory subunits of cAMP-dependent protein kinase A (PKA). PKA can be activated by cAMP. cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating PKA, which transduces the signal throughphosphorylation of different target proteins. The inactive holoenzyme of PKA is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of PKA have been identified in humans. PRKAR1A was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids Three alternatively spliced transcript variants encoding the same protein have been observed.

References

Huang L J. et al., 1997, Proc Natl Acad Sci. 94 (21): 11184-9. Herberg F W. et al., 2000, J Mol Biol. 298 (2): 329-39. Scambler P. et al., 1987, Am J Hum Genet. 41 (5): 925-32.

NCBI and Uniprot Product Information

• NCBI GI #: 30794476
• NCBI GeneID: 19084
• NCBI Accession #: NP_068680.1
• NCBI GenBank Nucleotide #: NM_021880.2
• UniProt Accession #: Q9DBC7; Q3UKU7; Q9JHR5; Q9JHR6
• Molecular Weight: 43,185 Da
• NCBI Official Full Name: cAMP-dependent protein kinase type I-alpha regulatory subunit
• NCBI Official Synonym Full Names: protein kinase, cAMP dependent regulatory, type I, alpha
• NCBI Official Symbol: Prkar1a
• NCBI Official Synonym Symbols: Tse1; Tse-1; RIalpha; 1300018C22Rik
• NCBI Protein Information: cAMP-dependent protein kinase type I-alpha regulatory subunit; protein kinase, cAMP dependent regulatory, type 1, alpha
• UniProt Protein Name: cAMP-dependent protein kinase type I-alpha regulatory subunit
• Protein Family: cAMP-dependent protein kinase
• UniProt Gene Name: Prkar1a
• UniProt Entry Name: KAP0_MOUSE