Human CNTF CLIA Kit | CNTF clia kit

CNTF (Human) LumiAb ELISA Kit

Cat. Num. AAA9510763

• Gene Names: CNTF; HCNTF
• Reactivity: Human
• Synonyms: CNTF; CNTF (Human) LumiAb ELISA Kit; Human CNTF; Ciliary neurotrophic factor; CNTF clia kit

• Reactivity: Human
• Specificity: The LumiAb Human CD163 ELISA is capable of recognizing both recombinant and naturally produced Human CD163 proteins. The antigens listed below were tested at 100 ng/ml and did not exhibit significant cross-reactivity or interference.
• Sequence Length: 200

• Assay Type: Quantitative Sandwich
• Sensitivity: 40 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CNTF clia kit

Principle of the Assay: The LumiAb Human CD163 Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human CD163 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human CD163 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: CD163, also known as Scavenger Receptor Cysteine-rich Type 1 Protein M130, is an 1156 amino acid secreted protein that is found on cell membranes. It is a single-pass type I membrane protein with isoform 1 and isoform 2 showing lower surface expression when expressed in cells. CD163 interacts with CSNK2B and is expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. It is also expressed in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood. CD 163 is induced by anti-inflammatory mediators such as glucocorticoids, interleukin-6/IL6 and interleukin-10/IL10; it is suppressed by proinflammatory mediators like bacterial LPS, IFN-gamma and TNF. CD163 functions as an acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and it may thereby protect tissues from free hemoglobin-mediated oxidative damage. CD163 binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner; it exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. CD163 induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. The SRCR domain 3 mediates calcium-sensitive interaction with hemoglobin/haptoglobin complexes. A soluble form (sCD163) of CD163 is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs. Intravenous LPS produces a rapid rise of sCD163 in plasma of patient as it induces metalloproteinase-mediated shedding from monocytes surface.

Product Categories/Family for CNTF clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 4758020
• NCBI GeneID: 1270
• NCBI Accession #: NP_000605.1
• NCBI GenBank Nucleotide #: NM_000614.4
• UniProt Accession #: P26441
• NCBI Official Full Name: ciliary neurotrophic factor
• NCBI Official Synonym Full Names: ciliary neurotrophic factor
• NCBI Official Symbol: CNTF
• NCBI Official Synonym Symbols: HCNTF
• NCBI Protein Information: ciliary neurotrophic factor
• UniProt Protein Name: Ciliary neurotrophic factor
• Protein Family: Ciliary neurotrophic factor
• UniProt Gene Name: CNTF
• UniProt Synonym Gene Names: CNTF
• UniProt Entry Name: CNTF_HUMAN

NCBI Description

The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of the upstream ZFP91 gene and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse. [provided by RefSeq, Oct 2010]

Uniprot Description

CNTF: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. Belongs to the CNTF family.

Chromosomal Location of Human Ortholog: 11q12.2

Cellular Component: extracellular space; cytoplasm

Molecular Function: interleukin-6 receptor binding; growth factor activity; ciliary neurotrophic factor receptor binding

Biological Process: negative regulation of photoreceptor cell differentiation; positive regulation of cell proliferation; regulation of retinal cell programmed cell death; neuron development; negative regulation of neuron apoptosis; signal transduction; muscle morphogenesis; growth; positive regulation of tyrosine phosphorylation of Stat3 protein