VCAM-1 / CD106 recombinant protein

Recombinant Human VCAM-1 / CD106 Protein (Fc tag)

Cat. Num. AAA2546515

• Gene Names: VCAM1; CD106; INCAM-100
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: VCAM-1 / CD106; Recombinant Human VCAM-1 / CD106 Protein (Fc tag); VCAM1; CD106; DKFZp779G2333; INCAM-100; MGC99561; VCAM-1 / CD106 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5
• Sequence Length: 739

• Application Notes: The recombinant human VCAM1/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 911 amino acids and has a predicted molecular mass of 101 kDa. As a result of glycosylation, the rh VCAM1/Fc monomer migrates as an approximately 130-140 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Phe 25
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for VCAM-1 / CD106 recombinant protein

Background: Vascular cell adhesion molecule 1 (VCAM-1), also known as CD106, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. Two forms of VCAM-1 with either six or seven extracellular Ig-like domains are generated by alternative splicing, with the longer form predominant. VCAM-1 is an endothelial ligand for very late antigen-4 (VLA-4) and alpha4beta7 integrin expressed on leukocytes, and thus mediates leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection, infection, and asthmatic responses. During these responses, VCAM-1 forms a scaffold for leukocyte migration. VCAM-1 also activates signals within endothelial cells resulting in the opening of an "endothelial cell gate" through which leukocytes migrate. VCAM-1 has been identified as a potential anti-inflammatory therapeutic target, the hypothesis being that reduced expression of VCAM-1 will slow the development of atherosclerosis. In addition, VCAM-1-activated signals in endothelial cells are regulated by cytokines indicating that it is important to consider both endothelial cell adhesion molecule expression and function during inflammatory processes.

Description: A DNA sequence encoding the extracellular domain (Met 1-Pro 697) of human VCAM1 (NP_001069.1) was expressed with the Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4507875
• NCBI GeneID: 7412
• NCBI Accession #: NP_001069.1
• NCBI GenBank Nucleotide #: NM_001078.3
• UniProt Accession #: P19320; Q6NUP8; A8K6R7; B4DKS4; E9PDD1
• Molecular Weight: 74,346 Da
• NCBI Official Full Name: vascular cell adhesion protein 1 isoform a
• NCBI Official Synonym Full Names: vascular cell adhesion molecule 1
• NCBI Official Symbol: VCAM1
• NCBI Official Synonym Symbols: CD106; INCAM-100
• NCBI Protein Information: vascular cell adhesion protein 1
• UniProt Protein Name: Vascular cell adhesion protein 1
• UniProt Gene Name: VCAM1
• UniProt Synonym Gene Names: L1CAM; V-CAM 1; VCAM-1

NCBI Description

This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

VCAM1: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1p21.2

Cellular Component: apical part of cell; cell surface; early endosome; endoplasmic reticulum; external side of plasma membrane; extracellular space; filopodium; Golgi apparatus; microvillus; plasma membrane; podosome

Molecular Function: amine oxidase activity; cell adhesion molecule binding; integrin binding

Biological Process: amine metabolic process; B cell differentiation; cell adhesion; cell-matrix adhesion; extracellular matrix organization and biogenesis; heterophilic cell adhesion; leukocyte adhesion; leukocyte tethering or rolling; membrane to membrane docking; positive regulation of T cell proliferation; regulation of immune response