Human Follistatin CLIA Kit | FST clia kit

Follistatin (Human) LumiAb ELISA Kit

Cat. Num. AAA9510771

• Gene Names: FST; FS
• Reactivity: Human
• Synonyms: Follistatin; Follistatin (Human) LumiAb ELISA Kit; Human Follistatin; FS; Activin-binding protein; FST clia kit

• Reactivity: Human
• Specificity: The LumiAb Human FGF-9 ELISA is capable of recognizing both recombinant and naturally produced Human FGF-9 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Sequence Length: 317

• Assay Type: Quantitative Sandwich
• Sensitivity: 15 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FST clia kit

Principle of the Assay: The LumiAb Human FGF-9 Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human FGF-9 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human FGF-9 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: The protein encoded by FGF-9 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis. Studies have shown that FGF-9 may have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors. Three molecular species were found (30 kDa, 29 kDa and 25 kDa), cleaved at Leu-4, Val-13 and Ser-34 respectively. The smaller ones might be products of proteolytic digestion. Furthermore, there may be a functional signal sequence in the 30 kDa species which is uncleavable in the secretion step. Defects in FGF9 are the cause of multiple synostoses syndrome type 3 (SYNS3). Multiple synostoses syndrome is an autosomal dominant condition characterized by progressive joint fusions of the fingers, wrists, ankles and cervical spine, characteristic facies and progressive conductive deafness.

Product Categories/Family for FST clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 5453652
• NCBI GeneID: 10468
• NCBI Accession #: NP_006341.1
• NCBI GenBank Nucleotide #: NM_006350.3
• UniProt Accession #: P19883
• NCBI Official Full Name: follistatin isoform FST317
• NCBI Official Synonym Full Names: follistatin
• NCBI Official Symbol: FST
• NCBI Official Synonym Symbols: FS
• NCBI Protein Information: follistatin
• UniProt Protein Name: Follistatin
• Protein Family: Follistatin
• UniProt Gene Name: FST
• UniProt Synonym Gene Names: FS
• UniProt Entry Name: FST_HUMAN

NCBI Description

Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin. [provided by RefSeq, Jul 2008]

Uniprot Description

FST: Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH). 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: cytoplasm; extracellular region; nucleus

Molecular Function: heparan sulfate proteoglycan binding; signal transducer activity; protein binding; activin binding

Biological Process: hair follicle morphogenesis; negative regulation of transcription from RNA polymerase II promoter; keratinocyte proliferation; pattern specification process; odontogenesis of dentine-containing teeth; BMP signaling pathway; positive regulation of hair follicle development; negative regulation of activin receptor signaling pathway; negative regulation of cell differentiation; gamete generation; hemopoietic progenitor cell differentiation; skeletal development; female gonad development

Disease: Polycystic Ovary Syndrome 1