Related Product Information for Gas6 clia kit
Principle of the Assay: The LumiAb Human Galectin-3 Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human Galectin-3 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human Galectin-3 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Galectin-3 is a galactose-specific lectin which binds IgE. This lectin may mediate with the alpha-3, beta-1 integrin for the stimulation by CSPG4 of endothelial cells migration. Found together with DMBT1, which is required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus, it acts as a pre-mRNA splicing factor. Galectin-3 is involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. It probably forms homo-or heterodimers. Additionally, galectin-3 interacts with DMBT1. This lectin forms a complex with ITGA3, ITGB1 and CSPG4. It also interacts with LGALS3BP, LYPD3, CYHR1 and UACA. It is highly expressed in the colonic epithelium and abundant in activated macrophages.
Product Categories/Family for Gas6 clia kit
NCBI Description
This gene encodes a gamma-carboxyglutamic acid (Gla)-containing protein thought to be involved in the stimulation of cell proliferation. This gene is frequently overexpressed in many cancers and has been implicated as an adverse prognostic marker. Elevated protein levels are additionally associated with a variety of disease states, including venous thromboembolic disease, systemic lupus erythematosus, chronic renal failure, and preeclampsia. [provided by RefSeq, Aug 2014]
Uniprot Description
GAS6: Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Ligand, receptor tyrosine kinase; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 13q34
Cellular Component: extracellular space; endoplasmic reticulum lumen; Golgi lumen; cytoplasm; extracellular region
Molecular Function: voltage-gated calcium channel activity; protein binding; phosphatidylserine binding; caspase inhibitor activity; protein tyrosine kinase activator activity; calcium ion binding; receptor tyrosine kinase binding; receptor agonist activity; molecular adaptor activity; receptor binding
Biological Process: proteolysis; enzyme linked receptor protein signaling pathway; protein amino acid phosphorylation; positive regulation of fibroblast proliferation; viral envelope fusion with host membrane; negative regulation of protein import into nucleus, translocation; regulation of growth; positive regulation of phagocytosis; negative regulation of interleukin-6 production; cell cycle arrest; cell adhesion; positive regulation of TOR signaling pathway; platelet activation; organ regeneration; activation of protein kinase B; negative regulation of transcription factor activity; cellular response to starvation; peptidyl-glutamic acid carboxylation; negative regulation of transcription, DNA-dependent; negative regulation of interleukin-1 secretion; leukocyte migration; negative regulation of apoptosis; entry of virus into host cell; neuron migration; negative regulation of caspase activity; signal transduction; positive regulation of protein export from nucleus; positive regulation of natural killer cell differentiation; platelet degranulation; positive regulation of glomerular filtration; negative regulation of interferon-gamma production; protein kinase B signaling cascade; virion attachment, binding of host cell surface receptor; cell migration; negative regulation of tumor necrosis factor production; viral genome replication; post-translational protein modification; positive regulation of peptidyl-serine phosphorylation; cell-substrate adhesion; phagocytosis; cell proliferation; positive regulation of protein kinase B signaling cascade; peptidyl-serine phosphorylation; cellular protein metabolic process; positive regulation of protein kinase activity; positive regulation of protein amino acid phosphorylation; blood coagulation; positive regulation of cytokine and chemokine mediated signaling pathway; apoptotic cell clearance
Product Notes
The Human Gas6 gas6 (Catalog #
AAA9510981) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9510981 CLIA Kit recognizes Human Gas6.
It is sometimes possible for the material contained within the vial of
"Gas6, CLIA Kit" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
suggest centrifuging these vials
to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
additional dry ice fee may also be
required.
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