Human Midkine CLIA Kit | MDK clia kit

Midkine (Human) LumiAb ELISA Kit

Cat. Num. AAA9510804

• Gene Names: MDK; MK; ARAP; NEGF2
• Reactivity: Human
• Synonyms: Midkine; Midkine (Human) LumiAb ELISA Kit; Human Midkine; MK; Amphiregulin-associated protein; ARAP; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein; MK1; NEGF2; MDK clia kit

• Reactivity: Human
• Specificity: The LumiAb Human Mesothelin ELISA is capable of recognizing both recombinant and naturally produced Human Mesothelin proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Sequence Length: 143

• Assay Type: Quantitative Sandwich
• Sensitivity: 32 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MDK clia kit

Principle of the Assay: The LumiAb Human Mesothelin Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human Mesothelin concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human Mesothelin while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Membrane-anchored forms of mesothelin may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. Mesothelin interacts with MUC16. The protein is expressed in lung and at low levels in the heart, placenta, kidney, and mesothelial cells. Mestothelin is highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). Both MPF and the cleaved form of mesothelin are N-glycosylated. It is proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.

Product Categories/Family for MDK clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 396080281
• NCBI GeneID: 4192
• NCBI Accession #: NP_001257480.1
• NCBI GenBank Nucleotide #: NM_001270551.1
• UniProt Accession #: P21741
• NCBI Official Full Name: midkine isoform a
• NCBI Official Synonym Full Names: midkine
• NCBI Official Symbol: MDK
• NCBI Official Synonym Symbols: MK; ARAP; NEGF2
• NCBI Protein Information: midkine
• UniProt Protein Name: Midkine
• Protein Family: Midkine
• UniProt Gene Name: MDK
• UniProt Synonym Gene Names: MK1; NEGF2; MK; ARAP
• UniProt Entry Name: MK_HUMAN

NCBI Description

This gene encodes a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. The encoded protein promotes cell growth, migration, and angiogenesis, in particular during tumorigenesis. This gene has been targeted as a therapeutic for a variety of different disorders. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2012]

Uniprot Description

MDK: Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development. Belongs to the pleiotrophin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: cell projection; cytoplasm; extracellular region

Molecular Function: heparin binding; growth factor activity

Biological Process: response to drug; nervous system development; cell migration; Notch signaling pathway; dentate gyrus development; behavioral fear response; cerebellar granular layer development; short-term memory; adrenal gland development; positive regulation of transcription, DNA-dependent; response to glucocorticoid stimulus; signal transduction; positive regulation of cell division; response to wounding; cerebral cortex development; negative regulation of neuron apoptosis; cell differentiation; defecation; regulation of behavior