PGLYRP1/PGRP-S active protein

Recombinant human PGLYRP1/PGRP-S protein

Cat. Num. AAA201349

• Gene Names: PGLYRP1; PGRP; TAG7; PGRPS; PGLYRP; PGRP-S; TNFSF3L
• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: PGLYRP1/PGRP-S; Recombinant human PGLYRP1/PGRP-S protein; PGLYRP; PGRP; PGRP-S; PGRPS; TAG7; TNFSF3L; Peptidoglycan recognition protein 1; peptidoglycan recognition protein short; PGLYRP1/PGRP-S active protein

• Host: HEK293
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 22-196aa
• Sequence: QETEDPA CCSPIVPRNE WKALASECAQ HLSLPLRYVV VSHTAGSSCN TPASCQQQAR NVQHYHMKTL GWCDVGYNFL IGEDGLVYEG RGWNFTGAHS GHLWNPMSIG ISFMGNYMDR VPTPQAIRAA QGLLACGVAQ GALRSNYVLK GHRDVQRTLS PGNQLYHLIQ NWPHYRSP

• Applicable Applications for PGLYRP1/PGRP-S active protein: SDS-PAGE, Bioactivity
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Human
• Tag: His-Tag
• Predicted N-term: Asp
• Bioactivity: Measured by its binding ability in a functional ELISA with Peptidoglycan. The ED50 range <= 30ng/ml.
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Biological Activity

(Peptidoglycan is coated at 10 ug/ml (100 ul/well) can bind Human PGLYRP1. The ED50 range )

Related Product Information for PGLYRP1/PGRP-S active protein

PGLYRP1, also known as peptidoglycan recognition protein 1, is an innate immunity protein that plays several important functions in antimicrobial and antitumor defense systems. It acts as a pattern receptor that binds to murein peptidoglycans of gram-positive bacteria and provides bactericidal activity. PGLYRP1 forms an equimolar complex with HSPA1A and induces programmed cell death through apoptosis and necroptosis in tumor cell lines by activating the TNFR1 receptor on the target cell membrane. This protein also interacts with S100A4 and function as a chmoattractant which induces lymphocyte movement. Recombinant human PGLYRP1, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Categories/Family for PGLYRP1/PGRP-S active protein

Apoptosis; Proteins

References

Lang MF, et al, (2008) Neurosci Lett. 430:138-141. Sharma P., et al, (2012) Int J Biochem Mol Biol. 3:86-94. Sun Q., et al, (2014) World J Microbiol Biotechnol. 30:263-270.

NCBI and Uniprot Product Information

• NCBI GI #: 4827036
• NCBI GeneID: 8993
• NCBI Accession #: NP_005082.1
• NCBI GenBank Nucleotide #: NM_005091.2
• UniProt Accession #: O75594; Q4VB36
• Molecular Weight: 21,731 Da
• NCBI Official Full Name: peptidoglycan recognition protein 1
• NCBI Official Synonym Full Names: peptidoglycan recognition protein 1
• NCBI Official Symbol: PGLYRP1
• NCBI Official Synonym Symbols: PGRP; TAG7; PGRPS; PGLYRP; PGRP-S; TNFSF3L
• NCBI Protein Information: peptidoglycan recognition protein 1; TNF superfamily, member 3 (LTB)-like (peptidoglycan recognition protein)
• UniProt Protein Name: Peptidoglycan recognition protein 1
• UniProt Gene Name: PGLYRP1
• UniProt Synonym Gene Names: PGLYRP; PGRP; TNFSF3L; PGRP-S
• UniProt Entry Name: PGRP1_HUMAN

Uniprot Description

PGLYRP1: Pattern receptor that binds to murein peptidoglycans (PGN) of Gram-positive bacteria. Has bactericidal activity towards Gram-positive bacteria. May kill Gram-positive bacteria by interfering with peptidoglycan biosynthesis. Binds also to Gram- negative bacteria, and has bacteriostatic activity towards Gram- negative bacteria. Plays a role in innate immunity. Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.

Protein type: Secreted, signal peptide; Apoptosis; Secreted; Receptor, misc.

Chromosomal Location of Human Ortholog: 19q13.2-q13.3

Cellular Component: extracellular region

Molecular Function: peptidoglycan receptor activity; peptidoglycan binding; zinc ion binding; N-acetylmuramoyl-L-alanine amidase activity

Biological Process: defense response to Gram-positive bacterium; negative regulation of natural killer cell differentiation during immune response; detection of bacterium; negative regulation of interferon-gamma production; negative regulation of inflammatory response; innate immune response; immune response; pattern recognition receptor signaling pathway; peptidoglycan catabolic process