CD47 active protein

Recombinant human CD47 protein

Cat. Num. AAA201328

• Gene Names: CD47; IAP; OA3; MER6
• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: CD47; Recombinant human CD47 protein; OA3; IAP; MER6; Leukocyte surface antigen CD47 isoform 1; antigenic surface determinant protein OA3; Integrin-associated protein; CD47 active protein

• Host: Baculovirus
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 1mg/ml (determined by absorbance at 280nm) (varies by lot)
• Sequence Positions: 19-141aa
• Sequence: QLLFNKTKSV EFTFCNDTVV IPCFVTNMEA QNTTEVYVKW KFKGRDIYTF DGALNKSTVP TDFSSAKIEV SQLLKGDASL KMDKSDAVSH TGNYTCEVTE LTREGETIIE LKYRVVSWFS PNE

• Applicable Applications for CD47 active protein: SDS-PAGE, Bioactivity
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Human
• Tag: His-Tag
• Predicted N-term: Gln19
• Bioactivity: Measured by its binding ability in a functional ELISA with Human SIRP gamma/CD172g
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. )

Biological Activity

(Human CD47 is coated at 1 ug/ml (100 ul/well) can bind Human SIRP gamma/CD172g (CAT# ATGP4030) in a Functional ELISA assay. )

Related Product Information for CD47 active protein

CD47, also known as leukocyte surface antigen CD47 isoform 1, has a role in both cell adhesions by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It plays an important role in memory formation and synaptic plasticity in the hippocampus. Receptor for SIRPA prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Recombinant human CD47, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for CD47 active protein

Immunology; Adhesion & Movement; Proteins

References

Iwamoto C et al., (2014) Exp. Hematol. 42(3):163-171. Wang Y et al., (2013) Mol. Ther. 21(10):1919-1929.

NCBI and Uniprot Product Information

• NCBI GI #: 4502673
• NCBI GeneID: 961
• NCBI Accession #: NP_001768
• NCBI GenBank Nucleotide #: NM_001777.3
• UniProt Accession #: Q08722; Q53Y71; Q96A60; A8K198; D3DN59
• Molecular Weight: 14.7kDa (129aa) 18-28KDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: leukocyte surface antigen CD47 isoform 1
• NCBI Official Synonym Full Names: CD47 molecule
• NCBI Official Symbol: CD47
• NCBI Official Synonym Symbols: IAP; OA3; MER6
• NCBI Protein Information: leukocyte surface antigen CD47
• UniProt Protein Name: Leukocyte surface antigen CD47
• Protein Family: Leukocyte surface antigen
• UniProt Gene Name: CD47
• UniProt Synonym Gene Names: MER6; IAP
• UniProt Entry Name: CD47_HUMAN

NCBI Description

This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

CD47: Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3q13.1-q13.2

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding

Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; response to bacterium; positive regulation of phagocytosis; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; positive regulation of T cell activation; opsonization; blood coagulation; cell adhesion; leukocyte migration; positive regulation of inflammatory response