PD-1 active protein

Recombinant human PD-1 protein

Cat. Num. AAA201340

• Gene Names: PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: PD-1; Recombinant human PD-1 protein; Programmed cell death protein 1; PDCD1; CD279; hPD-1; hPD-l; hSLE1; PD1; SLEB2; PD-1 active protein

• Host: HEK293
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 0.5mg/ml (determined by absorbance at 280nm) (varies by lot)
• Sequence Positions: 21-170aa
• Sequence: PGWFLDSPDR PWNPPTFSPA LLVVTEGDNA TFTCSFSNTS ESFVLNWYRM SPSNQTDKLA AFPEDRSQPG QDCRFRVTQL PNGRDFHMSV VRARRNDSGT YLCGAISLAP KAQIKESLRA ELRVTERRAE VPTAHPSPSP RPAGQFQTLV

• Applicable Applications for PD-1 active protein: SDS-PAGE, Bioactivity
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Human
• Tag: hIgG-Tag
• Predicted N-term: Pro21
• Bioactivity: Measured by its binding ability in a functional ELISA with Human PD-L1/B7-H1.
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Biological Activity

(Human PDCD1 is coated at 5 ug/ml (100 ul/well) can bind Human PD-L1/B7-H1 (CAT# ATGP3631) in a Functional ELISA assay.)

Related Product Information for PD-1 active protein

PD-1, also known as programmed cell death protein 1, is a type 1 transmembrane glycoprotein, and is an immune-receptor belonging to the CD28/CTLA4 family. This protein is expressed on the surface of activated T-cell, B-cell, macrophages, myeloid cells and subset of thymocytes. It has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self-tolerance by suppressing T cell inflammatory activity. This prevents autoimmune diseases, but it can also prevent the immune system from killing cancer cells. This protein is an immune checkpoint and guards against autoimmunity through two mechanisms. It promotes apoptosis of antigen-specific T-cells in lymph nodes, And reduces apoptosis in regulatory T cells. PD-1 inhibitors, a new class of drugs that block PD-1, activate the immune system to attack tumors and are used to treat certain types of cancer. Blocking the PD-1 and PD-L1 interaction has proven successful in treating many different cancer type. Recombinant human PD-1, fused to hIgG-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Categories/Family for PD-1 active protein

Immunology; Apoptosis; Proteins

References

Ishida Y., et al, (1992) EMBO J. 11:3887-3895. Lastwika KJ., et al, (2016) Cancer Res. 76:227-238.

NCBI and Uniprot Product Information

• NCBI GI #: 167857792
• NCBI GeneID: 5133
• NCBI Accession #: NP_005009.2
• NCBI GenBank Nucleotide #: NM_005018.2
• UniProt Accession #: Q15116; O00517; Q8IX89
• Molecular Weight: 31,647 Da
• NCBI Official Full Name: programmed cell death protein 1
• NCBI Official Synonym Full Names: programmed cell death 1
• NCBI Official Symbol: PDCD1
• NCBI Official Synonym Symbols: PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
• NCBI Protein Information: programmed cell death protein 1; protein PD-1; systemic lupus erythematosus susceptibility 2
• UniProt Protein Name: Programmed cell death protein 1
• UniProt Gene Name: PDCD1
• UniProt Synonym Gene Names: PD1; Protein PD-1; hPD-1

NCBI Description

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors.