OX40/TNFRSF4 active protein

Recombinant human OX40/TNFRSF4 protein

Cat. Num. AAA201358

• Gene Names: TNFRSF4; OX40; ACT35; CD134; IMD16; TXGP1L
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: OX40/TNFRSF4; Recombinant human OX40/TNFRSF4 protein; Tumor necrosis factor receptor superfamily member 4; TNFRSF4; ACT35; CD134; IMD16; OX40; TXGP1L; ACT35 antigen; OX40L receptor; TAX transcriptionally-activated glycoprotein 1 receptor; OX40/TNFRSF4 active protein

• Host: Baculovirus
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 0.5mg/ml (determined by absorbance at 280nm) (varies by lot)
• Sequence Positions: 29-214aa
• Sequence: LHCVGDTYPS NDRCCHECRP GNGMVSRCSR SQNTVCRPCG PGFYNDVVSS KPCKPCTWCN LRSGSERKQL CTATQDTVCR CRAGTQPLDS YKPGVDCAPC PPGHFSPGDN QACKPWTNCT LAGKHTLQPA SNSSDAICED RDPPATQPQE TQGPPARPIT VQPTEAWPRT SQGPSTRPVE VPGGRA

• Applicable Applications for OX40/TNFRSF4 active protein: SDS-PAGE, Bioactivity
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Human
• Tag: hIgG-His-Tag
• Predicted N-term: Leu29
• Bioactivity: Measured by its binding ability in a functional ELISA with mouse OX40 Ligand/TNFSF4. The ED50 range <= 0.15ug/ml.
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

Biological Activity

(Mouse OX40 Ligand/TNFSF4 (CAT# ATGP4022) is coated at 1 ug/ml (100 ul/well) can bind Human TNFRSF4. The ED50 range )

Related Product Information for OX40/TNFRSF4 active protein

TNFRSF4, also known as tumor necrosis factor receptor superfamily member 4, is a T cell co-stimulatory molecule in the TNF receptor superfamily. This protein coordinates with other co-stimulatory substances (CD28, CD40, CD30, CD27 and 4-1BB) to control the activation of the immune response. It plays an important role in antigen-specific T cell expansion and survival. It is up-regulated on CD4+ and CD8+ T cells upon engagement of the TCR by antigen presenting cells along with co-stimulation by CD40-CD40 Ligand and CD28-B7. This protein also regulates cytokine production from T cells, antigen presenting cells, natural killer cells and natural killer cells and regulate cytokine receptor signaling. Recombinant human TNFRSF4, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for OX40/TNFRSF4 active protein

Immunology; Proteins

References

Hori, T., et al. (2006) Int. J. Hematol. 83:17-22. Moran, A.E., et al. (2013) Curr. Opin. Immunol. 25:230-237.

NCBI and Uniprot Product Information

• NCBI GI #: 4507579
• NCBI GeneID: 7293
• NCBI Accession #: NP_003318.1
• NCBI GenBank Nucleotide #: NM_003327.3
• UniProt Accession #: P43489; Q13663; Q2M312; Q5T7M0
• Molecular Weight: 29,341 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 4
• NCBI Official Synonym Full Names: TNF receptor superfamily member 4
• NCBI Official Symbol: TNFRSF4
• NCBI Official Synonym Symbols: OX40; ACT35; CD134; IMD16; TXGP1L
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 4
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 4
• UniProt Gene Name: TNFRSF4
• UniProt Synonym Gene Names: TXGP1L
• UniProt Entry Name: TNR4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Knockout studies in mice suggested that this receptor promotes the expression of apoptosis inhibitors BCL2 and BCL2lL1/BCL2-XL, and thus suppresses apoptosis. The knockout studies also suggested the roles of this receptor in CD4+ T cell response, as well as in T cell-dependent B cell proliferation and differentiation. [provided by RefSeq, Jul 2008]

Uniprot Description

TNFRSF4: Receptor for TNFSF4/OX40L/GP34.

Protein type: Apoptosis; Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: cell surface; integral to plasma membrane; external side of plasma membrane

Molecular Function: tumor necrosis factor receptor activity

Biological Process: T cell proliferation; regulation of apoptosis; tumor necrosis factor-mediated signaling pathway; negative regulation of transcription factor activity; negative regulation of cytokine secretion; positive regulation of B cell proliferation; cellular defense response; immune response; positive regulation of immunoglobulin secretion; negative regulation of transcription, DNA-dependent; inflammatory response; regulation of protein kinase activity

Disease: Immunodeficiency 16