ULBP-6/RAET1L active protein

Recombinant human ULBP-6/RAET1L Protein

Cat. Num. AAA201336

• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: ULBP-6/RAET1L; Recombinant human ULBP-6/RAET1L Protein; early transcript 1L; ULBP6; UL16-binding protein 6;RAET1L; retinoic acid early transcript 1L protein; retinoic acid early transcript 1L; ULBP-6/RAET1L active protein

• Host: Baculovirus
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 1mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 26-218aa
• Sequence: RRDDPHS LCYDITVIPK FRPGPRWCAV QGQVDEKTFL HYDCGNKTVT PVSPLGKKLN VTMAWKAQNP VLREVVDILT EQLLDIQLEN YTPKEPLTLQ ARMSCEQKAE GHSSGSWQFS IDGQTFLLFD SEKRMWTTVH PGARKMKEKW ENDKDVAMSF HYISMGDCIG WLEDFLMGMD STLEPSAGAP LAMSSG

• Applicable Applications for ULBP-6/RAET1L active protein: SDS-PAGE, Bioactivity
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Human
• Tag: His-Tag
• Predicted N-term: Ala
• Bioactivity: Measured by its binding ability in a functional ELISA with Human NKG2D.
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain Lane 1 : reducing condition Lane 2 : non-reducing condition)

Biological Activity

(Human RAET1L/ULBP6 is coated at 10 ug/ml (100 ul/well) can bind Human NKG2D (CAT# ATGP3662) in a Functional ELISA assay.)

Related Product Information for ULBP-6/RAET1L active protein

RAET1L, also known as retinoic acid early transcript 1L, linked to the cell membrane by the GPI anchor. This protein functions as a stress-induced ligand for NKG2D receptor. Its expression is, for example, triggered in course of HCMV infection, but HCMV alters its function. HCMV-encoded UL16 glycoprotein retains RAET1L/ULBP6 inside the cells, preventing it from reaching the cell surface and being exposed to cells of the immune system. It had a more restricted expression pro?le in cell lines and primary human tissues than other NKG2D ligands. RAET1L mRNA shows restricted expression in the trachea and some HPV positive cervical carcinoma and colorectal carcinoma cell lines. Recombinant human RAET1L, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for ULBP-6/RAET1L active protein

Immunology; Proteins

References

Eagle R.A., et al. (2009) Eur. J. Immunol. 39:3207. Kondo M., et al. (2010) Immunogenetics 62:441.

NCBI and Uniprot Product Information

• UniProt Accession #: Q5VY80
• Molecular Weight: 22.9kDa (202aa)
• UniProt Protein Name: Retinoic acid early transcript 1L protein
• UniProt Gene Name: RAET1L
• UniProt Entry Name: RET1L_HUMAN

Uniprot Description

RAET1L: Belongs to the MHC class I family

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: plasma membrane

Molecular Function: peptide antigen binding; natural killer cell lectin-like receptor binding

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; natural killer cell mediated cytotoxicity