IL-17E/IL-25 active protein

Recombinant human IL-17E/IL-25 protein

Cat. Num. AAA201331

• Gene Names: IL25; IL17E
• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: IL-17E/IL-25; Recombinant human IL-17E/IL-25 protein; interleukin-25 isoform 1; interleukin 25; IL17E; IL-17E; IL25; IL-25; interleukin-17E; interleukin-25; IL-17E/IL-25 active protein

• Host: HEK293
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 20% glycerol
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 33-177aa
• Sequence: YSHWPSC CPSKGQDTSE ELLRWSTVPV PPLEPARPNR HPESCRASED GPLNSRAISP WRYELDRDLN RLPQDLYHAR CLCPHCVSLQ TGSHMDPRGN SELLYHNQTV FYRRPCHGEK GTHKGYCLER RLYRVSLACV CVRPRVMG

• Applicable Applications for IL-17E/IL-25 active protein: SDS-PAGE, Bioactivity
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Human
• Tag: His-Tag
• Predicted N-term: Asp
• Bioactivity: Measured by its binding ability in a functional ELISA with Human IL-17 RB
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain Lane 1 : reducing condition Lane 2 : non-reducing condition)

Biological Activity

(Human IL-17E/IL-25 is coated at 2 ug/ml (100 ul/well) can bind Human IL-17 RB (CAT# ATGP4039) in a Functional ELISA assay.)

Related Product Information for IL-17E/IL-25 active protein

IL-25, also known as IL-17E, belongs to the IL-17 cytokine family. It support the Th2 immune response, and has been shown to induce the production of IL-4, IL-5 and IL-13. This is in contrast to other IL-17 family members which promote Th1- and Th17-biased inflammation. Because IL-25 promotes the development of a Th2 immune response, it acts to protect against several bowel infections caused by helminths. It is also referred to as the regulator of IL-9 production. IL-25 has been shown to increase the production of IL-9 in Th9 cells. Another function of this protein is the activation of natural lymphoid cells. IL-25 and IL-33 are the most potent activators of ILC2. IL-25 has potent antitumor activity in vivo in several human cancers including melanoma, breast, lung, colon, and pancreatic cancers, suggesting the potential clinical use of IL-17E as an anticancer agent. Recombinant human IL-17E/IL-25, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Categories/Family for IL-17E/IL-25 active protein

Immunology; Hormones & Cytokines; Proteins

References

Xinyang Song., et al, (2013) Cell Signal. 25:2335-2347. Rickel EA., et al, (2008) J Immunol. 181:4299-4310.

NCBI and Uniprot Product Information

• NCBI GI #: 12232485
• NCBI GeneID: 64806
• NCBI Accession #: NP_073626.1
• NCBI GenBank Nucleotide #: NM_022789.3
• UniProt Accession #: Q9H293; Q2M3F0; Q8IZV3; Q8WXB0
• Molecular Weight: 18,523 Da
• NCBI Official Full Name: interleukin-25 isoform 1
• NCBI Official Synonym Full Names: interleukin 25
• NCBI Official Symbol: IL25
• NCBI Official Synonym Symbols: IL17E
• NCBI Protein Information: interleukin-25; interleukin-17E
• UniProt Protein Name: Interleukin-25
• UniProt Gene Name: IL25
• UniProt Synonym Gene Names: IL17E; IL-25; IL-17E
• UniProt Entry Name: IL25_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Uniprot Description

IL25: Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses. Belongs to the IL-17 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular space

Molecular Function: cytokine activity; interleukin-17E receptor binding

Biological Process: response to fungus; inflammatory response to antigenic stimulus; response to nematode; eosinophil differentiation; positive regulation of transcription from RNA polymerase II promoter