SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
CD200 active protein
Recombinant mouse CD200 protein
Applications
SDS-Page
Purity
>95% by SDS-PAGE
Synonyms
CD200; Recombinant mouse CD200 protein; Cd200; Mox2; OX2; OX-2 membrane glycoprotein isoform 1; MRC OX-2 antigen; CD200 active protein
Host
Baculovirus
Purity/Purification
>95% by SDS-PAGE
Form/Format
Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
Concentration
0.25mg/ml (determined by absorbance at 280nm) (varies by lot)
Sequence Positions
31-232aa
Sequence
QVEVVTQDER KALHTTASLR CSLKTSQEPL IVTWQKKKAV SPENMVTYSK THGVVIQPAY KDRINVTELG LWNSSITFWN TTLEDEGCYM CLFNTFGSQK VSGTACLTLY VQPIVHLHYN YFEDHLNITC SATARPAPAI SWKGTGTGIE NSTESHFHSN GTTSVTSILR VKDPKTQVGK EVICQVLYLG NVIDYKQSLD KG
Applicable Applications for CD200 active protein
SDS-PAGE, Bioactivity
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Species
Mouse
Tag
His-Tag
Predicted N-term
Gln31
Bioactivity
Measured by its binding ability in a functional ELISA with Mouse CD200 R1
Preparation and Storage
Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Biological Activity
(Mouse CD200 is coated at 1 ug/ml (100 ul/well) can bind CD200 R1 (CAT# ATGP3457) in a Functional ELISA assay.)
Biological Activity
(Mouse CD200 is coated at 1 ug/ml (100 ul/well) can bind CD200 R1 (CAT# ATGP3457) in a Functional ELISA assay.)
Related Product Information for CD200 active protein
CD200, also known as OX-2 membrane glycoprotein isoform 1, is one of the immunoglobulin superfamily. It plays an important role in anti-tumor immunity through interaction with its receptor, and overexpression of this protein effects on malignancies as well as on cancer stem cells. In T cells, this protein functions as a co-stimulatory effect that is independent of the CD28 pathway. It also plays an important role on myeloid cell regulation that is suggested by restricted expression of its receptor. Studies of this protein in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. Recombinant mouse CD200, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for CD200 active protein
References
Poongodi R., et al. (2018) Indian J Pathol Microbiol. 61:50-57. ;Kuwabara J., et al. (2018) Biochem Biophys Res Commun. 496:542-548.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
23.5kDa (210aa)
UniProt Protein Name
OX-2 membrane glycoprotein
Protein Family
UniProt Gene Name
Cd200
UniProt Synonym Gene Names
Mox2
UniProt Entry Name
OX2G_MOUSE
Uniprot Description
CD200: Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Cellular Component: membrane; integral to membrane; plasma membrane
Molecular Function: protein binding
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Product Notes
The CD200 cd200 (Catalog #AAA201277) is an Active Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 31-232aa. AAA Biotech's CD200 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Bioactivity. Researchers should empirically determine the suitability of the CD200 cd200 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QVEVVTQDER KALHTTASLR CSLKTSQEPL IVTWQKKKAV SPENMVTYSK THGVVIQPAY KDRINVTELG LWNSSITFWN TTLEDEGCYM CLFNTFGSQK VSGTACLTLY VQPIVHLHYN YFEDHLNITC SATARPAPAI SWKGTGTGIE NSTESHFHSN GTTSVTSILR VKDPKTQVGK EVICQVLYLG NVIDYKQSLD KGPrecautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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