Western Blot (WB)
(Western Blot detection of Sox2 antibody in F9 cell lysates using Sox2 antibody (1:1000 diluted).)
Mouse anti-Human, Mouse Sox2 Monoclonal Antibody | anti-Sox2 antibody
Sox2 Monoclonal Antibody
Gene Names
SOX2; ANOP3; MCOPS3
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
Sox2; Monoclonal Antibody; Sox2 Monoclonal Antibody; Transcription factor SOX-2; anti-Sox2 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Specificity
This antibody detects endogenous levels of Sox2 and does not cross-react with related proteins
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
317
Applicable Applications for anti-Sox2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
ICC: 1:50-200
FC/FACS: 1:50-100
ICC: 1:50-200
FC/FACS: 1:50-100
Immunogen
Recombinant full length Human Sox2.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot detection of Sox2 antibody in F9 cell lysates using Sox2 antibody (1:1000 diluted).)
Western Blot (WB)
(Western Blot detection of Sox2 antibody in F9 cell lysates using Sox2 antibody (1:1000 diluted).)
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis of F9 cells stained with Sox2 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.)
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis of F9 cells stained with Sox2 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.)
Immunocytochemistry (ICC)
(Immunocytochemistry of COS7 cells using anti-Sox2 antibody diluted 1:150.)
Immunocytochemistry (ICC)
(Immunocytochemistry of COS7 cells using anti-Sox2 antibody diluted 1:150.)
Related Product Information for anti-Sox2 antibody
SOX 2 is also known as SRY related HMG BOX gene 2. All SOX proteins have a single HMG box and bind linear DNA in a sequence-specific manner, resulting in the bending of DNA through large angles. Bending causes the DNA helix to open for some distance, which may affect binding and interactions of other transcription factors. SOX 2 is a transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted Band Size: 35KDa
Observed Band Size: 35KDa
Observed Band Size: 35KDa
NCBI Official Full Name
transcription factor SOX-2
NCBI Official Synonym Full Names
SRY-box transcription factor 2
NCBI Official Symbol
SOX2
NCBI Official Synonym Symbols
ANOP3; MCOPS3
NCBI Protein Information
transcription factor SOX-2
UniProt Protein Name
Transcription factor SOX-2
UniProt Gene Name
SOX2
UniProt Entry Name
SOX2_HUMAN
NCBI Description
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008]
Research Articles on Sox2
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Product Notes
The Sox2 sox2 (Catalog #AAA3014258) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Sox2 Monoclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Sox2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). WB: 1:1000 ICC: 1:50-200 FC/FACS: 1:50-100. Researchers should empirically determine the suitability of the Sox2 sox2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sox2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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