Testing Data
Eukaryotes DNAse I Assay Kit
DNAse I Activity Assay Kit (Fluorometric)
Reactivity
Eukaryotes
Synonyms
DNAse I; DNAse I Activity Assay Kit (Fluorometric); DNAse; DNAse I assay kit
Reactivity
Eukaryotes
Samples
Purified Protein, Serum
Detection Method
Fluorescence: Ex/Em 651/681nm
Features & Benefits
High-throughput compatible
Quick and easy screening for DNAse activity in biological samples; Includes DNAse positive control
Quantitative analysis of DNAse I mutants and inhibitors
Comparative examination of DNAse I activity in biological samples
Measurement of DNAse I activity of purified proteins
Quick and easy screening for DNAse activity in biological samples; Includes DNAse positive control
Quantitative analysis of DNAse I mutants and inhibitors
Comparative examination of DNAse I activity in biological samples
Measurement of DNAse I activity of purified proteins
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Testing Data
Testing Data
Related Product Information for DNAse I assay kit
Deoxyribonuclease I (DNAse I) is an endonuclease that cleaves DNA phosphodiester bonds yielding 5?-phosphorylated and 3?-hydroxylated oligonucleotides. DNAse I targets single-stranded DNA, double-stranded DNA, and chromatin in a non-specific manner. As an important player in cellular waste management, DNAse I is normally secreted extracellularly to clear the system from circulating cell-free DNA, foreign DNA from food digestion or potential pathogens, and endogenous chromosomal DNA from apoptotic and necrotic cells. Abnormal DNAse I activity occurs in association with a variety of cancers and auto-immune illnesses that exhibit elevated levels of cell-free DNA. Furthermore, DNAse I has been therapeutically used in cystic fibrosis patients to degrade DNA and reduce sputum viscosity. DNAse I Activity Fluorometric Assay Kit allows for quantitative evaluation of DNAse I activity of purified enzymes and their inhibitors as well as comparative examination of DNAse I activity in biological samples. Enzyme activity is detected upon cleavage of a DNA Probe, which yields a fluorescent DNA product measured at Ex/Em = 651/681 nm. The limit of quantification (L.O.Q) is 178 fmoles of DNA probe cleaved per minute per ml
Product Categories/Family for DNAse I assay kit
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Product Notes
The Eukaryotes DNAse I (Catalog #AAA849653) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849653 Assay Kit recognizes Eukaryotes DNAse I. It is sometimes possible for the material contained within the vial of "DNAse I, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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