OxiSelect UV-Induced DNA Damage (CPD Quantitation) ELISA Kit

OxiSelect UV-Induced DNA Damage ELISA Kit (CPD Quantitation)

Cat. Num. AAA168006

• Synonyms: OxiSelect UV-Induced DNA Damage (CPD Quantitation); OxiSelect UV-Induced DNA Damage ELISA Kit (CPD Quantitation); OxiSelect UV-Induced DNA Damage (CPD Quantitation) elisa kit

• Preparation and Storage: Upon receipt, Store the CPD-DNA standard at -20 degree C and all other components at 4 degree C until their expiration dates.

Typical Standard Curve/Testing Data

Related Product Information for OxiSelect UV-Induced DNA Damage (CPD Quantitation) elisa kit

Principle of the Assay: CDP-DNA standards or unknown DNA samples are first heat denatured before being adsorbed onto a 96-well DNA high-binding plate. The CPDs present in the sample or standard are probed with an anti-CPD antibody, followed by an HRP conjugated secondary antibody. The CPD content in an unknown sample is determined by comparing with a standard curve that is prepared from predetermined CPD-DNA standards.

Background/Introduction: Absorption of ultraviolet (UV) light produces two predominant types of DNA damage, cyclobutane pyrimidine dimers (CPD) and pyrimidine (6-4) pyrimidone photoproducts (6-4PP) (Figure 1). The result is a transition of C to T and CC to TT, which are the most frequent mutations of p53 in both human and mouse skin cancers. UV damaged DNA is usually repaired by nucleotide excision repair (NER) or base excision repair (BER). After UV exposure, cells activate p53 and stall the cell cycle for repair. If the damage is too severe, the cell will trigger apoptosis to get rid of DNA damaged, potentially mutant cells. OxiSelect Oxidative UV-induced DNA Damage ELISA Kit (CPD Quantitation) is an enzyme immunoassay developed for rapid detection and quantitation of CPD in any DNA samples. The quantity of CPD in unknown sample is determined by comparing its absorbance with that of a known CPD-DNA standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.

Product Categories/Family for OxiSelect UV-Induced DNA Damage (CPD Quantitation) elisa kit

Oxidative Stress/Damage; DNA/RNA Damage and Repair; UV Induced DNA Damage Kits

References

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