Typical Testing Data/Standard Curve (for reference only)
OxiSelect Oxidative DNA Damage (8-OHdG Quantitation) ELISA Kit
OxiSelect Oxidative DNA Damage ELISA Kit (8-OHdG Quantitation) (1 plate)
Synonyms
OxiSelect Oxidative DNA Damage (8-OHdG Quantitation); OxiSelect Oxidative DNA Damage ELISA Kit (8-OHdG Quantitation) (1 plate); OxiSelect Oxidative DNA Damage (8-OHdG Quantitation) elisa kit
Assay Type
Competitive (Quantitative)
Preparation and Storage
Upon receipt, aliquot and store the 8-OHdG Standard at -20 degree C and the 8-OHdG Conjugate at -80 degree C to avoid multiple freeze/thaw cycles. Store all other components at 4 degree C until their expiration dates.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for OxiSelect Oxidative DNA Damage (8-OHdG Quantitation) elisa kit
Background: Free radicals and other reactive species are constantly generated in vivo and cause oxidative damage to biomolecules, a process held in check only by the existence of multiple antioxidant and repair systems as well as the replacement of damaged nucleic acids, proteins and lipids. DNA is probably the most biologically significant target of oxidative attack, and it is widely thought that continuous oxidative damage to DNA is a significant contributor to the age-related development of the major cancers, such as those of the colon, breast, rectum, and prostate. Among numerous types of oxidative DNA damage, the formation of 8-hydroxydeoxyguanosine (8-OHdG) is a ubiquitous marker of oxidative stress. 8-OHdG, one of the oxidative DNA damage byproducts, is physiologically formed and enhanced by chemical carcinogens. During the repair of damaged DNA in vivo by exonucleases, the resulting 8-OH-dG is excreted without further metabolism into urine. The OxiSelect™ Oxidative DNA Damage ELISA Kit is a competitive enzyme immunoassay developed for rapid detection and quantitation of 8-OHdG in urine, serum, or other cell or tissue DNA samples. The quantity of 8-OHdG in unknown sample is determined by comparing its absorbance with that of a known 8-OHdG standard curve. The kit has an 8-OHdG detection sensitivity range of 100 pg/mL to 20 ng/mL. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.
Product Categories/Family for OxiSelect Oxidative DNA Damage (8-OHdG Quantitation) elisa kit
References
1. Patel P. R, Bevan R. J, Mistry N and Lunec J. (2007) J. Free Radic Biol Med. 42, 552-558.
2. Shen J, Deininger P, Hunt J. D, and Zhao H. (2007) Cancer 109, 574-580.
3. Wu L. L, Chiou C. C, Chang P. Y and Wu J. T. (2004) Clin Chim Acta. 339, 1-9
1. Keshari, K. R. et al. (2015). Noninvasive in vivo imaging of diabetes-induced renal oxidative stress and response to therapy using hyperpolarized 13C dehydroascorbate magnetic resonance. Diabetes. 64:344-352.
2. Glenn, D. J. et al. (2015). Cardiac steatosis potentiates angiotensin II effects in the heart. Am J Physiol Heart Circ Physiol. 308:H339-H350.
3. Attri, P. et al. (2015). Influence of reactive species on the modification of biomolecules generated from the soft plasma. Sci Rep. 5:8221.
4. Nakamura, K. et al. (2015). Bactericidal activity and mechanism of photo-irradiated polyphenols against Gram-positive and-negative bacteria. J Agric Food Chem. doi: 10.1021/jf5058588.
5. Kurgan, ?. et al. (2015). High sensitivity detection of salivary 8-hydroxy deoxyguanosine levels in patients with chronic periodontitis. J Periodontal Res. doi: 10.1111/jre.12263.
6. Macedo, R. C. S. et al. (2015). Effects of chronic resveratrol supplementation in military firefighters undergo a physical fitness test-a placebo-controlled, double blind study. Chem Biol Interact. 227:89-95.
7. Chen, G. et al. (2015). CYP2J2 overexpression attenuates nonalcoholic fatty liver disease induced by high-fat diet in mice. Am J Physiol Endocrinol Metab. 308:E97-E110.
8. Grek, C. L. et al. (2015). S-glutathionylation of buccal cell proteins as biomarkers of exposure to hydrogen peroxide. BBA Clinical. 2:31-39.
9. Hu, S. et al. (2015). Photo-protective effects of Oxyresveratrol and Kuwanon O on DNA damage induced by UVA in human epidermal keratinocytes. Chem Res Toxicol. doi: 10.1021/tx500497u.
10. Gimenez-Garzó, C. et al. (2015). Is cognitive impairment in cirrhotic patients due to increased peroxynitrite and oxidative stress? Antioxid Redox Signal. doi:10.1089/ars.2014.6240.
11. Kunwar, A., & Haston, C. K. (2014). Basal levels of glutathione peroxidase correlate with onset of radiation induced lung disease in inbred mouse strains. Am J Physiol Lung Cell Mol Physiol. 15:L597-604.
12. Ravikumar, P. et al. (2014). alpha-Klotho protects against oxidative damage in pulmonary epithelia. Am J Physiol Lung Cell Mol Physiol. 307:L566-575.
13. Weaver, A. C. et al. (2014). Efficacy of dietary spray dried plasma protein to mitigate the negative effects on performance of pigs fed diets with corn naturally contaminated with multiple mycotoxins. J Anim Sci. 9:3878-3886.
14. Mikula-Pietrasik, J. et al. (2014). Resveratrol derivative, 3,3',4,4'-tetrahydroxy-trans-stilbene, retards senescence of mesothelial cells via hormetic-like prooxidative mechanism. J Gerontol A Biol Sci Med Sci. 10.1093/gerona/glu172.
15. Changou, C. A. et al. (2014). Arginine starvation-associated atypical cellular death involves mitochondrial dysfunction, nuclear DNA leakage, and chromatin autophagy. Proc Natl Acad Sci U S A. 111:14147-14152.
16. Chung, K.W. et al. (2014). Molecular insights into SIRT1 protection against UVB-induced skin fibroblast senescence by suppression of oxidative stress and p53 acetylation. J Gerontol A Biol Sci Med Sci. 10.1093/gerona/glu137.
17. Singh, B. et al. (2014). Resveratrol inhibits estrogen-induced breast carcinogenesis through induction of NRF2-mediated protective pathways. Carcinogenesis. 10.1093/carcin/bgu120.
18. Jitschin, R. et al. (2014). Mitochondrial metabolism contributes to oxidative stress and reveals therapeutic targets in chronic lymphocytic leukemia. Blood 123:2663-2672.
19. Moore, J. et al. (2013). Protection of corneal epithelial stem cells prevents ultraviolet a damage during corneal collagen cross-linking treatment for Keratoconus. Br J Ophthalmol. 10.1136/bjophthalmol-2013-303816.
20. Kalghatgi, S. et al. (2013). Bactericidal antibiotic induce mitochondrial dysfunction and oxidative damage in mammalian cells. Science Translational Medicine. 5: 192ra85.
21. James, M.L. et al. (2013). VARA attenuates hyperoxia-induced impaired alveolar development and lung function in newborn mice. Am J Physiol Lung Cell Mol Physiol. 304:L803-L812.
22. Ravikumar, P. et al. (2013). Klotho protects lung epithelial cells against oxidative DNA damage. FASEB J. 27:722.3.
23. Singh, B. et al. (2013). MicroRNA-93 regulates NRF2 expression and is associated with breast carcinogenesis. Carcinogenesis. 10.1093/carcin/bgt026.
24. Singh, B. et al. (2012). Superoxide dismutase 3 is induced by antioxidants, inhibits oxidative dna damage and is associated with inhibition of estrogen-induced breast cancer. Carcinogenesis. 33:2601-2610.
25. Mercer, J.R. et al. (2012). The methyl xanthine caffeine inhibits DNA damage signaling and reactive species and reduces atherosclerosis in ApoE-/- mice. Arterioscler Thromb Vasc Biol. 32: 2461-2467.
26. Schutt, F. et al. (2012). Moderately reduced ATP levels promote oxidative stress and debilitate autophagic and phagocytic capacities in human RPE cells. Invest.Ophthalmol.Vis.Sci. 53:5354-5361.
27. Tanabe, K. et al. (2012). Nicorandil as a novel therapy for advanced diabetic nephropathy in the eNOS-deficient mouse. Am J Physiol Renal Physiol. 302: F1151-F1160.
28. Tzortzaki, E.G. et al. (2012). Oxidative DNA damage and somatic mutations: a link to the molecular pathogenesis of chronic inflammatory airway diseases. Chest. 141: 1243-1250.
29. Burnham, E.L. et al. (2012). Protandim does not influence alveolar epithelial permeability or intrapulmonary oxidative stress in human subjects with alcohol use disorders. Am J Physiol Lung Cell Mol Physiol. 302:L688-L699.
30. Singh, B. et al. (2012). Induction of NAD(P)H-quinone oxidoreductase 1 by antioxidants in female ACI rats is associated with decrease in oxidative DNA damage and inhibition of estrogen-induced breast cancer. Carcinogenesis 33:156-163.
31. Pialoux, V. et al. (2011). Losartan abolishes oxidative stress induced by intermittent hypoxia in humans. J. Physiol. 589:5529-5537.
32. Nguyen, H.L. et al. (2011). Oxidative stress and prostate cancer progression are elicited by membrane-type 1 matrix metalloproteinase. Mol. Cancer Res. 9:1305-1318.
33. Thompson, C.M. et al. (2011). Investigation of the mode of action underlying the tumorigenic response induced in B6C3F1 mice exposed orally to hexavalent chromium. Toxicol. Sci. 123:58-70.
34. Barsony, J. et al. (2011). Osteoclast response to low extracellular sodium and the mechanism of hyponatremia-induced bone loss. J. Biol. Chem. 286:10864-10875.
35. Li, M. et al. (2010). The ATM-p53 pathway suppresses aneuploidy-induced tumorigenesis. PNAS 107:14188-14193.
36. Hasegawa, T. et al. (2009). Suppression of nitrosative and oxidative stress to reduce cardiac allograft vasculopathy. Am.J. Physiol. Hear Circ. Physiol. 10.1152/ajpheart.00498.2008.
37. Pialoux, V. et al. (2009). Effects of exposure to intermittent hypoxia on oxidative stress and acute hypoxic ventilatory response in humans. Am. J. Respir. Crit. Care Med. 10.1164/rccm.200905-0671OC.
38. Ksiazek, K. et al. (2008). Impaired response to oxidative stress in senescent cells may lead to acccumulation of DNA damage in mesothelial cells from aged donors. Biochem. and Biophys. Res. Comm. 373:335-339.
39. Rao, M. et al. (2008). Mitochondrial DNA injury and mortality in hemodialysis patients. J. Am. Soc. Nephrol. 20:189-196.
40. Ksiazek, K. et al. (2008). Vulnerability to oxidative stress and different patterns of senescence in human peritoneal mesothelial cell strains. Am J. Physiol. Regulatory Integrative Comp. Physiol. 296:R374-R382.
2. Shen J, Deininger P, Hunt J. D, and Zhao H. (2007) Cancer 109, 574-580.
3. Wu L. L, Chiou C. C, Chang P. Y and Wu J. T. (2004) Clin Chim Acta. 339, 1-9
1. Keshari, K. R. et al. (2015). Noninvasive in vivo imaging of diabetes-induced renal oxidative stress and response to therapy using hyperpolarized 13C dehydroascorbate magnetic resonance. Diabetes. 64:344-352.
2. Glenn, D. J. et al. (2015). Cardiac steatosis potentiates angiotensin II effects in the heart. Am J Physiol Heart Circ Physiol. 308:H339-H350.
3. Attri, P. et al. (2015). Influence of reactive species on the modification of biomolecules generated from the soft plasma. Sci Rep. 5:8221.
4. Nakamura, K. et al. (2015). Bactericidal activity and mechanism of photo-irradiated polyphenols against Gram-positive and-negative bacteria. J Agric Food Chem. doi: 10.1021/jf5058588.
5. Kurgan, ?. et al. (2015). High sensitivity detection of salivary 8-hydroxy deoxyguanosine levels in patients with chronic periodontitis. J Periodontal Res. doi: 10.1111/jre.12263.
6. Macedo, R. C. S. et al. (2015). Effects of chronic resveratrol supplementation in military firefighters undergo a physical fitness test-a placebo-controlled, double blind study. Chem Biol Interact. 227:89-95.
7. Chen, G. et al. (2015). CYP2J2 overexpression attenuates nonalcoholic fatty liver disease induced by high-fat diet in mice. Am J Physiol Endocrinol Metab. 308:E97-E110.
8. Grek, C. L. et al. (2015). S-glutathionylation of buccal cell proteins as biomarkers of exposure to hydrogen peroxide. BBA Clinical. 2:31-39.
9. Hu, S. et al. (2015). Photo-protective effects of Oxyresveratrol and Kuwanon O on DNA damage induced by UVA in human epidermal keratinocytes. Chem Res Toxicol. doi: 10.1021/tx500497u.
10. Gimenez-Garzó, C. et al. (2015). Is cognitive impairment in cirrhotic patients due to increased peroxynitrite and oxidative stress? Antioxid Redox Signal. doi:10.1089/ars.2014.6240.
11. Kunwar, A., & Haston, C. K. (2014). Basal levels of glutathione peroxidase correlate with onset of radiation induced lung disease in inbred mouse strains. Am J Physiol Lung Cell Mol Physiol. 15:L597-604.
12. Ravikumar, P. et al. (2014). alpha-Klotho protects against oxidative damage in pulmonary epithelia. Am J Physiol Lung Cell Mol Physiol. 307:L566-575.
13. Weaver, A. C. et al. (2014). Efficacy of dietary spray dried plasma protein to mitigate the negative effects on performance of pigs fed diets with corn naturally contaminated with multiple mycotoxins. J Anim Sci. 9:3878-3886.
14. Mikula-Pietrasik, J. et al. (2014). Resveratrol derivative, 3,3',4,4'-tetrahydroxy-trans-stilbene, retards senescence of mesothelial cells via hormetic-like prooxidative mechanism. J Gerontol A Biol Sci Med Sci. 10.1093/gerona/glu172.
15. Changou, C. A. et al. (2014). Arginine starvation-associated atypical cellular death involves mitochondrial dysfunction, nuclear DNA leakage, and chromatin autophagy. Proc Natl Acad Sci U S A. 111:14147-14152.
16. Chung, K.W. et al. (2014). Molecular insights into SIRT1 protection against UVB-induced skin fibroblast senescence by suppression of oxidative stress and p53 acetylation. J Gerontol A Biol Sci Med Sci. 10.1093/gerona/glu137.
17. Singh, B. et al. (2014). Resveratrol inhibits estrogen-induced breast carcinogenesis through induction of NRF2-mediated protective pathways. Carcinogenesis. 10.1093/carcin/bgu120.
18. Jitschin, R. et al. (2014). Mitochondrial metabolism contributes to oxidative stress and reveals therapeutic targets in chronic lymphocytic leukemia. Blood 123:2663-2672.
19. Moore, J. et al. (2013). Protection of corneal epithelial stem cells prevents ultraviolet a damage during corneal collagen cross-linking treatment for Keratoconus. Br J Ophthalmol. 10.1136/bjophthalmol-2013-303816.
20. Kalghatgi, S. et al. (2013). Bactericidal antibiotic induce mitochondrial dysfunction and oxidative damage in mammalian cells. Science Translational Medicine. 5: 192ra85.
21. James, M.L. et al. (2013). VARA attenuates hyperoxia-induced impaired alveolar development and lung function in newborn mice. Am J Physiol Lung Cell Mol Physiol. 304:L803-L812.
22. Ravikumar, P. et al. (2013). Klotho protects lung epithelial cells against oxidative DNA damage. FASEB J. 27:722.3.
23. Singh, B. et al. (2013). MicroRNA-93 regulates NRF2 expression and is associated with breast carcinogenesis. Carcinogenesis. 10.1093/carcin/bgt026.
24. Singh, B. et al. (2012). Superoxide dismutase 3 is induced by antioxidants, inhibits oxidative dna damage and is associated with inhibition of estrogen-induced breast cancer. Carcinogenesis. 33:2601-2610.
25. Mercer, J.R. et al. (2012). The methyl xanthine caffeine inhibits DNA damage signaling and reactive species and reduces atherosclerosis in ApoE-/- mice. Arterioscler Thromb Vasc Biol. 32: 2461-2467.
26. Schutt, F. et al. (2012). Moderately reduced ATP levels promote oxidative stress and debilitate autophagic and phagocytic capacities in human RPE cells. Invest.Ophthalmol.Vis.Sci. 53:5354-5361.
27. Tanabe, K. et al. (2012). Nicorandil as a novel therapy for advanced diabetic nephropathy in the eNOS-deficient mouse. Am J Physiol Renal Physiol. 302: F1151-F1160.
28. Tzortzaki, E.G. et al. (2012). Oxidative DNA damage and somatic mutations: a link to the molecular pathogenesis of chronic inflammatory airway diseases. Chest. 141: 1243-1250.
29. Burnham, E.L. et al. (2012). Protandim does not influence alveolar epithelial permeability or intrapulmonary oxidative stress in human subjects with alcohol use disorders. Am J Physiol Lung Cell Mol Physiol. 302:L688-L699.
30. Singh, B. et al. (2012). Induction of NAD(P)H-quinone oxidoreductase 1 by antioxidants in female ACI rats is associated with decrease in oxidative DNA damage and inhibition of estrogen-induced breast cancer. Carcinogenesis 33:156-163.
31. Pialoux, V. et al. (2011). Losartan abolishes oxidative stress induced by intermittent hypoxia in humans. J. Physiol. 589:5529-5537.
32. Nguyen, H.L. et al. (2011). Oxidative stress and prostate cancer progression are elicited by membrane-type 1 matrix metalloproteinase. Mol. Cancer Res. 9:1305-1318.
33. Thompson, C.M. et al. (2011). Investigation of the mode of action underlying the tumorigenic response induced in B6C3F1 mice exposed orally to hexavalent chromium. Toxicol. Sci. 123:58-70.
34. Barsony, J. et al. (2011). Osteoclast response to low extracellular sodium and the mechanism of hyponatremia-induced bone loss. J. Biol. Chem. 286:10864-10875.
35. Li, M. et al. (2010). The ATM-p53 pathway suppresses aneuploidy-induced tumorigenesis. PNAS 107:14188-14193.
36. Hasegawa, T. et al. (2009). Suppression of nitrosative and oxidative stress to reduce cardiac allograft vasculopathy. Am.J. Physiol. Hear Circ. Physiol. 10.1152/ajpheart.00498.2008.
37. Pialoux, V. et al. (2009). Effects of exposure to intermittent hypoxia on oxidative stress and acute hypoxic ventilatory response in humans. Am. J. Respir. Crit. Care Med. 10.1164/rccm.200905-0671OC.
38. Ksiazek, K. et al. (2008). Impaired response to oxidative stress in senescent cells may lead to acccumulation of DNA damage in mesothelial cells from aged donors. Biochem. and Biophys. Res. Comm. 373:335-339.
39. Rao, M. et al. (2008). Mitochondrial DNA injury and mortality in hemodialysis patients. J. Am. Soc. Nephrol. 20:189-196.
40. Ksiazek, K. et al. (2008). Vulnerability to oxidative stress and different patterns of senescence in human peritoneal mesothelial cell strains. Am J. Physiol. Regulatory Integrative Comp. Physiol. 296:R374-R382.
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Product Notes
The OxiSelect Oxidative DNA Damage (8-OHdG Quantitation) (Catalog #AAA168191) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "OxiSelect Oxidative DNA Damage (8-OHdG Quantitation), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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