OxiSelect Comet Assay Kit

OxiSelect Comet Assay Kit (3-Well Slides)

Cat. Num. AAA168415

• Synonyms: OxiSelect Comet; OxiSelect Comet Assay Kit (3-Well Slides); OxiSelect Comet assay kit

• Assay Type: Quantitative Sandwich
• Preparation and Storage: Upon receipt, store the Vista Green DNA Dye at -20 degree C. Store all other kit components at room temperature until their expiration dates.

Testing Data

Testing Data #2

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Related Product Information for OxiSelect Comet assay kit

Principle of the Assay: The OmniKine Rat IL-1beta ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Rat IL-1beta concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Rat IL-1beta while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Rat IL-1beta, also known as Interleukin-1beta, is a 268 amino acid cytokine protein encoded by the Il1b gene located at locus 3q36 on chromosome 3. After initial synthesis, it undergoes proteolytic processing to remove the 116 residue propeptide sequence from the 152 residue IL-1alpha peptide, thus allowing for proper folding and maturation. Produced by activated macrophages, IL-1beta stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1beta proteins, monomeric members of the IL-1 family, are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1beta is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Product Categories/Family for OxiSelect Comet assay kit

Oxidative Stress/Damage; DNA/RNA Damage and Repair; Comet Assay Kits and Slides

References

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