OxiSelect Comet Assay Kit

OxiSelect Comet Assay Kit (3-Well Slides)

Cat. Num. AAA168757

• Synonyms: OxiSelect Comet; OxiSelect Comet Assay Kit (3-Well Slides); OxiSelect Comet assay kit

• Preparation and Storage: Upon receipt, store the Vista Green DNA Dye at -20 degree C. Store all other kit components at room temperature until their expiration dates.

Testing Data

Testing Data #3

Related Product Information for OxiSelect Comet assay kit

Principle of the Assay: OxiSelect™ Comet Assay is a single cell gel electrophoresis assay (SCGE) for simple evaluation of cellular DNA damage. First, individual cells are mixed with molten agarose before application to the OxiSelect™ Comet Slide. These embedded cells are then treated with a lysis buffer and alkaline solution, which relaxes and denatures the DNA. Finally, the samples are electrophoresed in a horizontal chamber to separate intact DNA from damaged fragments. Following electrophoresis, the samples are dried, stained with a DNA dye, and visualized by epifluorescence microscopy. Under these conditions, the damaged DNA (containing cleavage and strand breaks) will migrate further than intact DNA and produce a "comet tail" shape (see Figure 1). Each kit provides sufficient reagents to perform up to 15 assays.

Background/Introduction: DNA damage, due to environmental factors and normal metabolic processes inside the cell, occurs at a rate of 1,000 to 1,000,000 molecular lesions per cell per day. While this counts for only a small part of the human genome's approximately 6 billion bases (3 billion base pairs), unrepaired lesions to critical genes can impede a cell's ability to carry out its function and appreciably increase the likelihood of cancer. The comet assay, or single cell gel electrophoresis assay (SCGE), is a common technique for measurement of DNA damage in individual cells. Under an electrophoretic field, damaged cellular DNA (containing fragments and strand breaks) is separated from intact DNA, yielding a classic "comet tail" shape under the microscope. Extent of DNA damage is usually visually estimated by comet tail measurement; however, image analysis software is also available for measuring various parameters. The OxiSelect™ Comet Assay is a fast and sensitive kit for the measurement of cellular DNA damage. Each kit provides sufficient reagents to perform up to 15 assays.

Product Categories/Family for OxiSelect Comet assay kit

Oxidative Stress/Damage; DNA/RNA Damage and Repair; Comet Assay Kits and Slides

References

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