Testing Data
OxiSelect TBARS Assay Kit
OxiSelect TBARS Assay Kit (MDA Quantitation)
Synonyms
OxiSelect TBARS; OxiSelect TBARS Assay Kit (MDA Quantitation); OxiSelect TBARS assay kit
Preparation and Storage
Store all components at 4 degree C until their expiration dates.
Testing Data
Testing Data
Testing Data #2
Testing Data #2
Related Product Information for OxiSelect TBARS assay kit
Principle of the Assay: The Thiobarbituric Acid Reactive Substances (TBARS) Assay Kit is a tool for the direct quantitative measurement of MDA in biological samples. The unknown MDA containing samples or MDA standards are first reacted with TBA at 95 degree C. After a brief incubation, the samples and standards can be read either spectrophotometrically or fluorometrically. The MDA content in unknown samples is determined by comparison with the predetermined MDA standard curve.
Background/Introduction: Lipid peroxidation is a well-defined mechanism of cellular damage in animals and plants. Lipid peroxides are unstable indicators of oxidative stress in cells that decompose to form more complex and reactive compounds such as Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), natural bi-products of lipid peroxidation. Oxidative modification of lipids can be induced in vitro by a wide array of pro-oxidant agents and occurs in vivo during aging and in certain disease conditions. Measuring the end products of lipid peroxidation is one of the most widely accepted assays for oxidative damage. These aldehydic secondary products of lipid peroxidation are generally accepted markers of oxidative stress. Thiobarbituric Acid Reactive Substances (TBARS) is a well-established assay for screening and monitoring lipid peroxidation. The rapid and easy protocol has been modified by researchers in the evaluation of drugs, food, as well as human and animal tissue samples. MDA forms a 1:2 adduct with thiobarbituric acid (Figure 1). The MDA-TBA adduct formed from the reaction of MDA in samples with TBA can be measured colorimetrically or fluorometrically. TBARS levels are determined from a Malondialdehyde equivalence standard. The TBARS Assay has provided relevant information concerning free radical activity in disease states and measurement of many compounds anti-oxidant characteristics. Although the specificity of TBARS toward compounds other than MDA has been controversial, the assay continues to be the most widely employed format for monitoring lipid peroxidation. Lipids with higher degrees of unsaturated bonds produce higher TBARS values. Interfering soluble TBARS can be minimized if lipoprotein fractions are first acid precipitated from samples. Biological samples may contain a mixture of thiobarbituric acid reactive substances such as hydroperoxides and aldehydes, which increase in response to oxidative stress. If excessively high TBARS values are obtained, a more specific assay such as HPLC should be employed. The OxiSelect™ TBARS Assay Kit offers a simple, reproducible, and consistent system for the detection of lipid peroxidation in urine, plasma, serum, lysates, and tissue homogenates. This kit includes an MDA standard for use as a positive control. Each kit provides sufficient reagents to perform 200 tests including standard curve and unknown samples.
Background/Introduction: Lipid peroxidation is a well-defined mechanism of cellular damage in animals and plants. Lipid peroxides are unstable indicators of oxidative stress in cells that decompose to form more complex and reactive compounds such as Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), natural bi-products of lipid peroxidation. Oxidative modification of lipids can be induced in vitro by a wide array of pro-oxidant agents and occurs in vivo during aging and in certain disease conditions. Measuring the end products of lipid peroxidation is one of the most widely accepted assays for oxidative damage. These aldehydic secondary products of lipid peroxidation are generally accepted markers of oxidative stress. Thiobarbituric Acid Reactive Substances (TBARS) is a well-established assay for screening and monitoring lipid peroxidation. The rapid and easy protocol has been modified by researchers in the evaluation of drugs, food, as well as human and animal tissue samples. MDA forms a 1:2 adduct with thiobarbituric acid (Figure 1). The MDA-TBA adduct formed from the reaction of MDA in samples with TBA can be measured colorimetrically or fluorometrically. TBARS levels are determined from a Malondialdehyde equivalence standard. The TBARS Assay has provided relevant information concerning free radical activity in disease states and measurement of many compounds anti-oxidant characteristics. Although the specificity of TBARS toward compounds other than MDA has been controversial, the assay continues to be the most widely employed format for monitoring lipid peroxidation. Lipids with higher degrees of unsaturated bonds produce higher TBARS values. Interfering soluble TBARS can be minimized if lipoprotein fractions are first acid precipitated from samples. Biological samples may contain a mixture of thiobarbituric acid reactive substances such as hydroperoxides and aldehydes, which increase in response to oxidative stress. If excessively high TBARS values are obtained, a more specific assay such as HPLC should be employed. The OxiSelect™ TBARS Assay Kit offers a simple, reproducible, and consistent system for the detection of lipid peroxidation in urine, plasma, serum, lysates, and tissue homogenates. This kit includes an MDA standard for use as a positive control. Each kit provides sufficient reagents to perform 200 tests including standard curve and unknown samples.
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References
1. Armstrong, D. and Browne, R. (1994). Free Radicals in Diagnostic Medicine. 366: 43-58.
2. Armstrong, D., et al. (1998). Free Radicals and Antioxidant Protocols. 108: 315-324.
3. Boyum, A. (1966). J. of Clinical Investigation. 21: Supplement 97.
4. Braun, D. and Fromherz, P. (1997). Applied Physics A.5. Gidez, L., et al. (1982). J. of Lipid Research. 23: 1206-1223.
6. Lef'evre G., et al. (1998). Annals de Biologie Clinique. 56(3): 305-319.
7. Ohkawa, H., et al. (1979). Anal. Biochem. 95: 351-358.
8. Yagi, K. (1998). Free Radicals and Antioxidant Protocols. 108: 101-106.
1. Fu, L. et al. (2012). Ethyl Pyruvate Reduces Ventilation-Induced Neutrophil Infiltration and Oxidative Stress. J. Lipid Res. : 1080-1092.
2. Brindeiro, C.M. et al.(2012).Tempol Prevents Altered K+ Channel Regulation of Afferent Arteriolar Tone in Diabetic Rat Kidney. Hypertension.59:657 - 664.
3. Joshi, S. G. et al. (2011). Nonthermal Dielectric-Barrier Discharge Plasma-Induced Inactivation Involves Oxidative DNA Damage and Memebrane Lipid Peroxidation in Escherichia coli. Antimicrob. Agents Chemother. 55:1053-1062.
4. Kasaikina, M.V. et al. (2011). Roles of the 15-kDa Selenoprotein (Sep15) in Redox Homeostasis and Cataract Development Revealed by the Analysis of Sep 15 Knockout Mice. J. Biol. Chem. 286:33203-33212.
5. Fedeles, B. I. et al. (2011). Chemical Genetics Analysis of an Aniline Mustard Anticancer Agent Reveals Complex I of the Electron Transport Chain as a Target. J. Biol. Chem. 286:33910-33920.
6. Song, Y.R. et al. (2010). Activation of Hypoxia-Inducible Factor Attenuates Renal Injury in Rat Remnant Kidney. Nephrol. Dial. Transplant. 25:77-85.
7. Wojciechowski, P. et al. (2010). Resveratrol Arrests and Regresses the Development of Pressure Overload- but Not Volume Overload-Induced Cardiac Hypertrophy in Rats. J. Nutr. 140:962-968.
8. Kwon, H. et al. (2010). Vitamin D3 Upregulated Protein 1 Suppresses TNF--Induced NF-B Activation in Hepatocarcinogenesis. J. Immunol. 185:3980-3989.
9. Fomenko, D.E. et al. (2008). Methionine-R-sulfoxide reductase 1 (MsrB1) Knockout Mice: Roles of MsrB1 in Redox Regulation and Identification of a Novel Selenoprotein form. J. Biol. Chem. 10.1074/jbc.M805770200.
10. Fujita, K. et al. (2008). Effectiveness of Antiplatelet Drugs Against Experimental Non-Alcoholic Fatty Liver Disease. Gut 57:1583-1591.
2. Armstrong, D., et al. (1998). Free Radicals and Antioxidant Protocols. 108: 315-324.
3. Boyum, A. (1966). J. of Clinical Investigation. 21: Supplement 97.
4. Braun, D. and Fromherz, P. (1997). Applied Physics A.5. Gidez, L., et al. (1982). J. of Lipid Research. 23: 1206-1223.
6. Lef'evre G., et al. (1998). Annals de Biologie Clinique. 56(3): 305-319.
7. Ohkawa, H., et al. (1979). Anal. Biochem. 95: 351-358.
8. Yagi, K. (1998). Free Radicals and Antioxidant Protocols. 108: 101-106.
1. Fu, L. et al. (2012). Ethyl Pyruvate Reduces Ventilation-Induced Neutrophil Infiltration and Oxidative Stress. J. Lipid Res. : 1080-1092.
2. Brindeiro, C.M. et al.(2012).Tempol Prevents Altered K+ Channel Regulation of Afferent Arteriolar Tone in Diabetic Rat Kidney. Hypertension.59:657 - 664.
3. Joshi, S. G. et al. (2011). Nonthermal Dielectric-Barrier Discharge Plasma-Induced Inactivation Involves Oxidative DNA Damage and Memebrane Lipid Peroxidation in Escherichia coli. Antimicrob. Agents Chemother. 55:1053-1062.
4. Kasaikina, M.V. et al. (2011). Roles of the 15-kDa Selenoprotein (Sep15) in Redox Homeostasis and Cataract Development Revealed by the Analysis of Sep 15 Knockout Mice. J. Biol. Chem. 286:33203-33212.
5. Fedeles, B. I. et al. (2011). Chemical Genetics Analysis of an Aniline Mustard Anticancer Agent Reveals Complex I of the Electron Transport Chain as a Target. J. Biol. Chem. 286:33910-33920.
6. Song, Y.R. et al. (2010). Activation of Hypoxia-Inducible Factor Attenuates Renal Injury in Rat Remnant Kidney. Nephrol. Dial. Transplant. 25:77-85.
7. Wojciechowski, P. et al. (2010). Resveratrol Arrests and Regresses the Development of Pressure Overload- but Not Volume Overload-Induced Cardiac Hypertrophy in Rats. J. Nutr. 140:962-968.
8. Kwon, H. et al. (2010). Vitamin D3 Upregulated Protein 1 Suppresses TNF--Induced NF-B Activation in Hepatocarcinogenesis. J. Immunol. 185:3980-3989.
9. Fomenko, D.E. et al. (2008). Methionine-R-sulfoxide reductase 1 (MsrB1) Knockout Mice: Roles of MsrB1 in Redox Regulation and Identification of a Novel Selenoprotein form. J. Biol. Chem. 10.1074/jbc.M805770200.
10. Fujita, K. et al. (2008). Effectiveness of Antiplatelet Drugs Against Experimental Non-Alcoholic Fatty Liver Disease. Gut 57:1583-1591.
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Product Notes
The OxiSelect TBARS (Catalog #AAA168658) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "OxiSelect TBARS, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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