Typical Testing Data/Standard Curve (for reference only)
Poly (ADP-Ribose) ELISA Kit
Poly (ADP-Ribose) ELISA Kit
Synonyms
Poly (ADP-Ribose); Poly (ADP-Ribose) ELISA Kit; Poly (ADP-Ribose) elisa kit
Assay Type
Quantitative Sandwich
Preparation and Storage
Upon receipt, aliquot store the Poly (ADP-Ribose) Standard at -80 degree C and the antibodies at 20 degree C. Avoid multiple freeze/thaw cycles. Store all other components at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Poly (ADP-Ribose) elisa kit
Principle of the Assay: The Poly (ADP-ribose) ELISA kit is a sandwich ELISA for the quantitative measurement of PAR. The samples or PAR standards are first added to a monoclonal anti-PAR antibody coated microplate. After a brief incubation, an anti-PAR polyclonal detection antibody is added, followed by an HRP conjugated secondary antibody. After adding the substrate a colorimetric reaction takes place that can be measured with a colorimetric plate reader. The PAR content in unknown samples is determined by comparing this absorbance with the ones from the PAR polymer standard curve.
Background/Introduction: Poly ADP-ribosylation (PAR), the addition of several ADP-ribose groups to proteins, is a post-translational modification in eukaryotic cells that modulates the activity of the modified proteins and their interaction with other ones. This modification is mediated by poly (ADP-ribose) polymerases (PARPs) and ADP-ribosyltransferase 2 (ART2) that use nicotinamide adenine dinucleotide (NAD) as a substrate. ADP-ribosylation can be reversed by PAR-degrading enzymes like poly (ADP-ribose) glycohydrolase (PARG) and ADP-ribosylhydrolase 3 (ARH3) (Figure 1). Poly ADP-ribosylation is involved in different biological functions like carcinogenesis, differentiation and cell death. Dysregulation of ADP-ribosylation activity has been implicated in the pathogenesis of diseases like cancer, virus infections, and neurodegeneration. One of the most studied roles of ADP-ribosylation is the generation of PAR chains in response to DNA damage. These PAR chains are used to recruit repair proteins and chromatin remodelers. In consequence, DNA repair processes are enhanced. This could be a mechanism for tumors to escape apoptosis. Recently, several inhibitors of PARP family members have shown promise in treating certain cancers, and three drugs are commercially available in the US and Europe, mainly for the treatment of BRCA-mutant ovarian cancer. Poly (ADP-ribose) ELISA Kit is an enzyme immunoassay developed for rapid detection and quantitation of PAR levels in cell or tissue samples. The quantity of PAR in an unknown sample is determined by comparing its absorbance with that of a known standard curve. The kit has a detection sensitivity limit of 20 pM. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.
Background/Introduction: Poly ADP-ribosylation (PAR), the addition of several ADP-ribose groups to proteins, is a post-translational modification in eukaryotic cells that modulates the activity of the modified proteins and their interaction with other ones. This modification is mediated by poly (ADP-ribose) polymerases (PARPs) and ADP-ribosyltransferase 2 (ART2) that use nicotinamide adenine dinucleotide (NAD) as a substrate. ADP-ribosylation can be reversed by PAR-degrading enzymes like poly (ADP-ribose) glycohydrolase (PARG) and ADP-ribosylhydrolase 3 (ARH3) (Figure 1). Poly ADP-ribosylation is involved in different biological functions like carcinogenesis, differentiation and cell death. Dysregulation of ADP-ribosylation activity has been implicated in the pathogenesis of diseases like cancer, virus infections, and neurodegeneration. One of the most studied roles of ADP-ribosylation is the generation of PAR chains in response to DNA damage. These PAR chains are used to recruit repair proteins and chromatin remodelers. In consequence, DNA repair processes are enhanced. This could be a mechanism for tumors to escape apoptosis. Recently, several inhibitors of PARP family members have shown promise in treating certain cancers, and three drugs are commercially available in the US and Europe, mainly for the treatment of BRCA-mutant ovarian cancer. Poly (ADP-ribose) ELISA Kit is an enzyme immunoassay developed for rapid detection and quantitation of PAR levels in cell or tissue samples. The quantity of PAR in an unknown sample is determined by comparing its absorbance with that of a known standard curve. The kit has a detection sensitivity limit of 20 pM. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.
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Product Notes
The Poly (ADP-Ribose) (Catalog #AAA169573) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Poly (ADP-Ribose), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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