OxiSelect Oxidative DNA Damage (AP sites) Assay Kit

OxiSelect Oxidative DNA Damage Quantitation Kit (AP sites)

Cat. Num. AAA168105

• Synonyms: OxiSelect Oxidative DNA Damage (AP sites); OxiSelect Oxidative DNA Damage Quantitation Kit (AP sites); OxiSelect Oxidative DNA Damage (AP sites) assay kit

• Preparation and Storage: Upon receipt, aliquot and store both the Reduced DNA and ARP-DNA Standards at -20 degree C to avoid multiple freeze/thaw cycles. Store all other components at 4 degree C until their expiration dates.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for OxiSelect Oxidative DNA Damage (AP sites) assay kit

Background/Introduction: Free radicals and other reactive species are constantly generated in vivo and cause oxidative damage to biomolecules, a process held in check only by the existence of multiple antioxidant and repair systems as well as the replacement of damaged lipids and proteins. DNA is probably the most biologically significant target of oxidative attack, and it is widely thought that continuous oxidative damage to DNA is a significant contributor to the age-related development of the major cancers, such as those of the colon, breast, rectum, and prostate. Among numerous types of oxidative DNA damage, apurinic/apyrimidinic (AP or abasic) site is one of the prevalent lesions of oxidative DNA damage. Abasic sites arise in DNA at a significant rate by spontaneous base loss as in depurination, by DNA oxidation, or by the action of DNA glycosylases. Estimates of the number of abasic sites generated per mammalian cell run as high as 50,000 to 200,000 per day. Unrepaired abasic sites inhibit topoisomerases, replication, and transcription and can be mutagenic because of bypass synthesis on nontemplated DNA. The OxiSelect™ Oxidative DNA Damage Quantitation Kit (AP sites) uses an Aldehyde Reactive Probe (ARP) to react specifically with an aldehyde group on the open ring form of AP sites. This allows for the AP sites to be tagged with biotin which is later detected with Streptavidin-Enzyme conjugate. The quantity of AP sites in unknown DNA sample is determined by comparing its absorbance with a standard curve generated from the provided DNA standard containing predetermined AP sites. The kit has a detection sensitivity range of 4 to 40 AP sites per 1 x 105 bp. Each kit provides sufficient reagents to perform up to 50 assays for unknown samples, excluding the standard curve.

Product Categories/Family for OxiSelect Oxidative DNA Damage (AP sites) assay kit

Oxidative Stress/Damage; DNA/RNA Damage and Repair; AP Sites Quantitation Kit

References

1. Croteau D L, Bohr V A. (1997) J Biol Chem. 272:25409-25412.
2. Lindahl T. (1993) Nature 362:709-715.
3. Kubo K, Ide H, Wallace S S, Kow Y W. (1992) Biochemistry 31:3703-3708.
1. Ferreira, E. et al. (2015). Glyceraldehyde-3-Phosphate Dehydrogenase is required for efficient repair of cytotoxic DNA lesions in Escherichia coli. Int J Biochem Cell Biol. 60:202-212.
2. Zhao, K. et al. (2014). S-sulfhydration of MEK1 leads to PARP-1 activation and DNA damage repair. EMBO Rep. 15:792-800.
3. Mohammad, M. K. et al. (2014). Watermelon (Citrullus lanatus (Thunb.) Matsum. and Nakai) juice modulates oxidative damage induced by low dose X-ray in mice. Biomed Res Int. 2014:512834.
4. Zafiropoulos, A. et al. (2014). Cardiotoxicity in rabbits after a low-level exposure to diazinon, propoxur, and chlorpyrifos. Hum Exp Toxicol. 33:1241-1252.
5. Messaoudi, N. et al. (2013). Global stress response in a prokaryotic model of DJ-1-associated Parkinsonism. J.Bacteriol. 195:1167-1178.
6. Zaika, E. et al. (2011). p73 protein regulates DNA damage repair. FASEB J. 25:4406-4414.