Principle of the assay: BSA standards or protein samples (10 mug/mL) are adsorbed onto a 96-well plate for 2 hrs at 37 degree C. The BPDE-protein adducts present in the sample or standard are probed with an Anti-BPDE-I Antibody, followed by an HRP Conjugated Secondary Antibody. The BPDE protein adduct content in an unknown sample is determined by comparing with a standard curve that is prepared from predetermined BPDE-BSA standards.
Background: Polycyclic aromatic hydrocarbons (PAHs) are potent, ubiquitous atmospheric pollutants commonly associated with oil, coal, cigarette smoke, and automobile exhaust fumes. Some PAH compounds are also found in cooked foods (e.g. grilled meat, smoked fish) and have been identified as mutagenic and carcinogenic. The toxicity of some PAHs has been demonstrated to induce malignant tumors in animal models and is also commonly believed to significantly contribute to human cancers. One PAH compound, benzo(a)pyrene, is notable for being the first chemical carcinogen to be discovered. Benzo(a)pyrene is a five-ring PAH known to be a procarcinogen; its mechanism of carcinogenesis is dependent on a 3-step enzymatic metabolism (Fig. 1 below) to the final mutagen benzo(a)pyrene diol epoxide (BPDE). Very reactive, BPDE binds covalently to proteins, lipids, and DNA (guanine residues) to produce BPDE adducts. If left unrepaired, DNA adducts may lead to permanent mutations resulting in cell transformation and ultimately tumor development. The OxiSelect BPDE Protein Adduct ELISA Kit is an enzyme immunoassay developed for rapid detection of BPDE-protein adducts. The quantity of BPDE adduct in protein samples is determined by relative comparison of a known BPDE-BSA standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples.
2. Denissenko MF, Pao A, Tang M, Pfeifer GP (1996) Science 274:430-432.
3. Lee, B.M. and Santella, R.M. (1988) Carcinogenesis, 9: 1773-1777.
4. Tang D, Phillips DH, Stampfer M, Mooney LA, Hsu Y, Cho S, Tsai WY, Ma J, Cole KJ, She MN, Perera FP. (2001) Cancer Research 61:6708-6712.
5. Mumford JL, Williams K, Wilcosky TC, Everson RB, Young TL, Santella RM. (1996) Mutat Res. 359:171-177.
6. Santella RM, Perera FP, Young TL, Zhang YJ, Chiamprasert S, Tang D, Wang LW, Beachman A, Lin JH, DeLeo VA. (1995) Mutation Research 334:117-124.
7. Chung MK, Riby J, Li H, Iavarone AT, Williams ER, Zheng Y, Rappaport SM. (2010) Anal. Biochem. 400:123-129.
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Product Notes
The OxiSelect BPDE Protein Adduct (Catalog #AAA168495) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "OxiSelect BPDE Protein Adduct, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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