Typical Testing Data/Standard Curve (for reference only)
OxiSelect Protein Carbamylation ELISA Kit
OxiSelect Protein Carbamylation Sandwich ELISA Kit
Synonyms
OxiSelect Protein Carbamylation; OxiSelect Protein Carbamylation Sandwich ELISA Kit; OxiSelect Protein Carbamylation elisa kit
Preparation and Storage
Upon receipt, aliquot and store the CBL-BSA Standard at -20 degree C to avoid multiple freeze/thaw cycles. Store all other kit components at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for OxiSelect Protein Carbamylation elisa kit
Principle of the Assay: Caspase (Cysteine-requiring Aspartate Protease) is a protease family that plays an important role in the process of apoptosis. Caspase 9 (also known as ICE-LAP6, or mch6) is considered to be a caspase in the upstream of apoptotic signal transduction. When mitochondria release cytochrome c, Caspase 9 can form complex with cytochrome c and Apaf1, and be activated at the same time. Activated Caspase 9 can activate Caspase 3 which is the key enzyme of cell apoptosis, thereby promoting subsequent apoptotic signals. Caspase 9 exists in the form of prozyme in the normal state and has no activity. However, Caspase 9 is activated in the apoptotic stage and participates in the apoptotic process. This kit is used to conjugate Caspase 9 sequence-specific peptides acetyl-Leu-Glu-His-Asp p-nitroanilide (AcLEHD-pNA) to yellow group p-nitroaniline (pNA). When the substrate is cut by Caspase 9, the yellow group pNA is dissociated. pNA has an absorption peak at 405nm. Measure the OD value at 405 nm and then Caspase 9 activity can be calculated accordingly!!Background/Introduction: The Caspase 9 Activity Assay Kit can detect the activity of Caspase 9 in cells or tissue lysis.
Product Categories/Family for OxiSelect Protein Carbamylation elisa kit
References
1. Wang, Z., et al. (2007). Nat. Med. 13:1176-1184.
2. Kraus, L.M., et al. (2001). Swiss Med. Wkly. 131:139-144
3. Sirpal, S. (2009). Clin. Sci. (Lond.) 116:681-695.
4. Balion, C.M., et al. (1998). Kidney Int. 53:488-495.
5. Asci, G., et al. (2008). Nephrology (Carlton) 13:480-486.
6. Jaisson, S., et al. (2007). FEBS Lett. 581:1509-1513.
7. Garnotel, R., et al. (2004). FEBS Lett. 563:13-16
2. Kraus, L.M., et al. (2001). Swiss Med. Wkly. 131:139-144
3. Sirpal, S. (2009). Clin. Sci. (Lond.) 116:681-695.
4. Balion, C.M., et al. (1998). Kidney Int. 53:488-495.
5. Asci, G., et al. (2008). Nephrology (Carlton) 13:480-486.
6. Jaisson, S., et al. (2007). FEBS Lett. 581:1509-1513.
7. Garnotel, R., et al. (2004). FEBS Lett. 563:13-16
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Product Notes
The OxiSelect Protein Carbamylation (Catalog #AAA168597) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "OxiSelect Protein Carbamylation, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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