Typical Testing Data/Standard Curve (for reference only)
OxiSelect AOPP Assay Kit
OxiSelect AOPP Assay Kit (200 assays)
Synonyms
OxiSelect AOPP; OxiSelect AOPP Assay Kit (200 assays); OxiSelect AOPP assay kit
Preparation and Storage
Upon receipt, store the AOPP-HSA positive control at -20 degree C. Store all other components at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for OxiSelect AOPP assay kit
Principle of the assay: The Advanced Oxidation Protein Products (AOPP) Assay Kit is a bioassay tool for the direct quantitative measurement of AOPPs in biological samples. The unknown AOPP-containing samples or Chloramine standards are first mixed with an assay reaction initiator that begins a color development process. After a brief incubation, a stop solution is added and the samples and standards can be read with a standard colorimetric plate reader. The AOPP content in unknown samples is determined by comparison with the predetermined Chloramine standard curve.
Background: Oxidative stress is defined as an increase in the production of reactive oxygen species (ROS) due to an imbalance between antioxidant and oxidants. Advanced Oxidation Protein Products (AOPP) are uremic toxins created during oxidative stress through the reaction of chlorinated oxidants, such as chloramines and hypochlorous acid, with plasma proteins. AOPPs are structurally similar to Advanced Glycation End-Product (AGE) proteins and exert similar biological activities. AOPPs are elevated in patients with renal complications, atherosclerosis, diabetes mellitus, systemic sclerosis, as well as HIV-positive patients. Human Serum Albumin (HSA) treated with HOCl and AOPP generated in vivo can ignite oxidative reactions in both neutrophils and monocytes, which indicates both can be used as true mediators of inflammation. Although the mechanisms of AOPP degradation and elimination from the blood remain to be fully elucidated, it appears that the liver and spleen are mostly responsible for their isolation and removal. The AOPP Assay has provided relevant information concerning free radical activity in many uremic associated disease states and the measurement of anti-oxidant characteristics of many compounds. The rapid and easy protocol has been modified by researchers in the evaluation of plasma and tissue samples. The AOPP-HSA concentration was determined from a Chloramine equivalence standard. The OxiSelect AOPP Assay Kit offers a simple, reproducible, and consistent system for the detection of advanced oxidation protein products in plasma, lysates, and tissue homogenates. This kit includes a Chloramine standard and an AOPP Human Serum Albumin conjugate for use as a positive control. Each kit provides sufficient reagents to perform 200 tests including standard curve and unknown samples.
Product Categories/Family for OxiSelect AOPP assay kit
References
1. Deschamps-Latscha B. et al. (2005) Am. J. Kidney Dis. 45(1): 39-47.
2. Servettaz, A. et al. (2007) Ann. Rheum. Dis. 66: 1202-1209.
3. Witko-Sarsat, V. et al. (1996) Kidney International 49: 1304-1313.
2. Servettaz, A. et al. (2007) Ann. Rheum. Dis. 66: 1202-1209.
3. Witko-Sarsat, V. et al. (1996) Kidney International 49: 1304-1313.
Similar Products
Product Notes
The OxiSelect AOPP (Catalog #AAA168459) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "OxiSelect AOPP, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
