Typical Testing Data/Standard Curve (for reference only)
OxiSelect Nepsilon-(carboxyethyl) lysine (CEL) ELISA Kit
OxiSelect Nepsilon-(carboxyethyl) lysine (CEL) Competitive ELISA Kit
Synonyms
OxiSelect Nepsilon-(carboxyethyl) lysine (CEL); OxiSelect Nepsilon-(carboxyethyl) lysine (CEL) Competitive ELISA Kit; OxiSelect Ne-(carboxyethyl) lysine (CEL) Competitive ELISA Kit; OxiSelect Nepsilon-(carboxyethyl) lysine (CEL) elisa kit
Preparation and Storage
Upon receipt, aliquot and store the Anti-CEL Antibody, CEL-BSA Standard, CEL Conjugate and 100X Conjugate Diluent at -20 degree C to avoid multiple freeze/thaw cycles. Store all other kit components at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for OxiSelect Nepsilon-(carboxyethyl) lysine (CEL) elisa kit
Principle of the assay: First, a CEL conjugate is coated on the ELISA plate. The unknown CEL protein samples or CEL-BSA standards are then added to the CEL conjugate preabsorbed plate. After a brief incubation, the anti-CEL monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The content of CEL protein adducts in unknown samples is determined by comparison with the predetermined CEL-BSA standard curve. Despite the structure similarity between CEL and CML, the anti-CEL specific antibody in the OxiSelect CEL ELISA Kit will not cross react with CML protein adducts.
Background: The non-enzymatic reaction of reducing carbohydrates with lysine side chains and N-terminal amino groups of macromolecules (proteins, phospholipids and nucleic acids) is called the Maillard reaction or glycation. The products of this process, termed advanced glycation end products (AGEs), adversely affect the functional properties of proteins, lipids and DNA. Tissue levels of AGE increase with age and the formation of AGEs is predominantly endogenous, though these products can also be derived from exogenous sources such as food and tobacco smoke. AGE modification of proteins can contribute to the pathophysiology of aging and long-term complications of diabetes, atherosclerosis and renal failure. AGEs also interact with a variety of cell-surface AGE-binding receptors (RAGE), leading either to their endocytosis and degradation or to cellular activation and pro-oxidant or pro-inflammatory events. Although several AGE structures have been reported, it was demonstrated that Nepsilon-(carboxymethyl) lysine (CML) and Nepsilon-(carboxyethyl) lysine (CEL) are the major antigenic AGE structures. Next to glucose, reactive di-carbonyl compounds such as methylglyoxal are major precursors in the formation of cellular and extracellular AGEs. Methylglyoxal reacts with lysine residues to form CEL. CEL concentration is increased in patients who have diabetes with complications. OxiSelect Nepsilon-(carboxyethyl) lysine (CEL) ELISA Kit provides rapid detection and quantitation of CEL protein adducts. The quantity of CEL adduct in protein samples is determined by comparing its absorbance with that of a known CEL-BSA standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples.
Product Categories/Family for OxiSelect Nepsilon-(carboxyethyl) lysine (CEL) elisa kit
References
1. Monnier, V., and Cerami, A. (1981) Science 211, 491-493.
2. Ahmed M.U., Thorpe S.R., Baynes J.W (1986) J. Biol. Chem. 261, 4889-4894.
3. Reddy S., Bichler J., Wells-Knecht K.J., Thorpe S.R., Baynes J.W (1995) Biochemistry 34, 10872-10878.
4. Dunn, J. A., Patrick, J. S., Thorpe, S. R., and Baynes, J. W. (1989) Biochemistry 28, 9464-9468.
5. Ahmed, M. U., Brinkmann Frye, E., Degenhardt, T. P., Thorpe, S. R., and Baynes, J. W. (1997) Biochem. J. 324, 565-570.
6. Sell, D. R., and Monnier, V. M. (1989) J. Biol. Chem. 264, 21597-21602.
7. Onorato, J., Jenkins, A., Thorpe, S., and Baynes, J. (2000) J. Biol. Chem. 275, 21177-21184.
8. Boehm BO, Schilling S, Rosinger S, Lang GE, Lang GK, Kientsch-Engel P, Stahl P (2004) Diabetologia 47, 1376-1379.
9. Koito W, Araki T, Horiuchi S, Nagai R (2004) J. Biochem. 136, 831-837.
10. Nagai R., Fujiwara Y., Mera K., Yamagata K., Sakashita N., Takeya M. (2008) J. Immunol. Methods 332, 112-120.
Morgan, P. E. et al. (2014). Perturbation of human coronary artery endothelial cell redox state and NADPH generation by methylglyoxal. PLoS One. 9:e86564.
2. Ahmed M.U., Thorpe S.R., Baynes J.W (1986) J. Biol. Chem. 261, 4889-4894.
3. Reddy S., Bichler J., Wells-Knecht K.J., Thorpe S.R., Baynes J.W (1995) Biochemistry 34, 10872-10878.
4. Dunn, J. A., Patrick, J. S., Thorpe, S. R., and Baynes, J. W. (1989) Biochemistry 28, 9464-9468.
5. Ahmed, M. U., Brinkmann Frye, E., Degenhardt, T. P., Thorpe, S. R., and Baynes, J. W. (1997) Biochem. J. 324, 565-570.
6. Sell, D. R., and Monnier, V. M. (1989) J. Biol. Chem. 264, 21597-21602.
7. Onorato, J., Jenkins, A., Thorpe, S., and Baynes, J. (2000) J. Biol. Chem. 275, 21177-21184.
8. Boehm BO, Schilling S, Rosinger S, Lang GE, Lang GK, Kientsch-Engel P, Stahl P (2004) Diabetologia 47, 1376-1379.
9. Koito W, Araki T, Horiuchi S, Nagai R (2004) J. Biochem. 136, 831-837.
10. Nagai R., Fujiwara Y., Mera K., Yamagata K., Sakashita N., Takeya M. (2008) J. Immunol. Methods 332, 112-120.
Morgan, P. E. et al. (2014). Perturbation of human coronary artery endothelial cell redox state and NADPH generation by methylglyoxal. PLoS One. 9:e86564.
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Product Notes
The OxiSelect Nepsilon-(carboxyethyl) lysine (CEL) (Catalog #AAA169081) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "OxiSelect Nepsilon-(carboxyethyl) lysine (CEL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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