OxiSelect Nitrotyrosine ELISA Kit

OxiSelect Nitrotyrosine ELISA Kit (1 plate)

Cat. Num. AAA169017

• Synonyms: OxiSelect Nitrotyrosine; OxiSelect Nitrotyrosine ELISA Kit (1 plate); OxiSelect Nitrotyrosine elisa kit

• Preparation and Storage: Upon receipt, aliquot and store the Nitrated BSA Standard at -20 degree C to avoid multiple freeze/thaw cycles. Store all other kit components at 4 degree C until their expiration dates.

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Related Product Information for OxiSelect Nitrotyrosine elisa kit

Principle of the assay: The nitrotyrosine quantitation kit is a competitive ELISA. The unknown protein nitrotyrosine sample or nitrated BSA standards are first added to a nitrated BSA preabsorbed EIA plate. After a brief incubation, an anti-nitrotyrosine antibody is added, followed by an HRP conjugated secondary antibody. The protein nitrotyrosine content in unknown sample is determined by comparing with a standard curve that is prepared from predetermined nitrated BSA standards.

Background: The modification of tyrosine residues in proteins to 3-nitrotyrosine by peroxynitrite (Figure 1) or other potential nitrating agents has been detected in biological systems that are subject to oxidative stress. Detection of nitrotyrosine-containing proteins has been reported in many human and animal diseases or cellular models of disease. While all tyrosine residues in proteins may theoretically be targets for nitration, presumably the efficiency of tyrosine nitration is dependent on various biological conditions such as the local production and concentration of the reactive species, the existence and availability of antioxidants and scavengers, the accumulation of inflammatory cell and the presence of pro-inflammatory cytokines, as well as the proximity and compartmentation of these components. The OxiSelect Nitrotyrosine ELISA Kit is a competitive enzyme immunoassay developed for rapid detection and quantitation of 3-nitrotyrosine in protein sample. The quantity of 3-nitrotyrosine in protein sample is determined by comparing its absorbance with that of a known nitrated BSA standard curve. The kit has a nitrotyrosine detection sensitivity range of 20 nM to 8.0 muM. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples.

Product Categories/Family for OxiSelect Nitrotyrosine elisa kit

Oxidative Stress/Damage; Protein Oxidation/Nitration; Protein Nitration Assays and Reagents

References

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