Typical Testing Data/Standard Curve (for reference only)
OxiSelect Methylglyoxal (MG) ELISA Kit
OxiSelect Methylglyoxal (MG) Competitive ELISA Kit
Synonyms
OxiSelect Methylglyoxal (MG); OxiSelect Methylglyoxal (MG) Competitive ELISA Kit; OxiSelect Methylglyoxal (MG) elisa kit
Preparation and Storage
Upon receipt, aliquot and store the Anti-MG Antibody, MG-BSA Standard, MG Conjugate and 100X Conjugate Diluent at -20 degree C to avoid multiple freeze/thaw cycles. Store all other kit components at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for OxiSelect Methylglyoxal (MG) elisa kit
Principle of the assay: First, an MG conjugate is coated on the ELISA plate. The unknown MG protein samples or MG-BSA standards are then added to the MG conjugate preabsorbed plate. After a brief incubation, the anti-MG antibody is added, followed by an HRP conjugated secondary antibody. The content of MG protein adducts in unknown samples is determined by comparison with the predetermined MG-BSA standard curve.
Background: The non-enzymatic reaction of reducing carbohydrates with lysine side chains and N-terminal amino groups of macromolecules (proteins, phospholipids and nucleic acids) is called the Maillard reaction or glycation. The products of this process, termed advanced glycation end products (AGEs), adversely affect the functional properties of proteins, lipids and DNA. Tissue levels of AGE increase with age and the formation of AGEs is predominantly endogenous, though these products can also be derived from exogenous sources such as food and tobacco smoke. AGE modification of proteins can contribute to the pathophysiology of aging and long-term complications of diabetes, atherosclerosis and renal failure. AGEs also interact with a variety of cell-surface AGE-binding receptors (RAGE), leading either to their endocytosis and degradation or to cellular activation and pro-oxidant or pro-inflammatory events. Several AGE structures have been reported, such as Nepsilon-(carboxymethyl) lysine (CML), Nepsilon-(carboxyethyl) lysine (CEL), pentosidine, and Methylglyoxal (MG) derivatives. MG is formed through non-oxidative mechanisms from triose phosphates during anaerobic glycolysis and it can modify amino acids, nucleic acids, and proteins. MG reacts with arginine, lysine and cysteine residues of proteins to form AGEs. MG is involved in various pathological processes. For example, MG derivatives are found elevated in diabetes. The OxiSelect Methylglyoxal (MG) ELISA Kit is an enzyme immunoassay developed for rapid detection and quantitation of MG-H1 (methyl-glyoxal-hydro-imidazolone) protein adducts. The quantity of MG adduct in protein samples is determined by comparing its absorbance with that of a known MG-BSA standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples.
Product Categories/Family for OxiSelect Methylglyoxal (MG) elisa kit
References
1. Shamshi F. A, Partal A, Sady C, Glomb M. A, Nagaraj R. H (1998) J Biol Chem 273:6928-6936.
2. Cai W, Gao Q. D, Zhu L, Peppa M, He C, Vlassara H. (2002), Mol Med. 8:337-46.
3. Monnier, V., and Cerami, A. (1981) Science 211, 491-493.
4. Ahmed M.U., Thorpe S.R., Baynes J.W (1986) J. Biol. Chem. 261, 4889-4894.
5. Reddy S., Bichler J., Wells-Knecht K.J., Thorpe S.R., Baynes J.W (1995) Biochemistry 34, 10872-10878.
6. Dunn, J. A., Patrick, J. S., Thorpe, S. R., and Baynes, J. W. (1989) Biochemistry 28, 9464-946
7. Ahmed, M. U., Brinkmann Frye, E., Degenhardt, T. P., Thorpe, S. R., and Baynes, J. W. (1997) Biochem. J. 324, 565-570.
8. Sell, D. R., and Monnier, V. M. (1989) J. Biol. Chem. 264, 21597-21602.
9. Onorato, J., Jenkins, A., Thorpe, S., and Baynes, J. (2000) J. Biol. Chem. 275, 21177-21184.
10. Boehm BO, Schilling S, Rosinger S, Lang GE, Lang GK, Kientsch-Engel P, Stahl P (2004) Diabetologia 47, 1376-1379.
Morgan, P. E. et al. (2014). Perturbation of human coronary artery endothelial cell redox state and NADPH generation by methylglyoxal. PLoS One. 9:e86564.
2. Cai W, Gao Q. D, Zhu L, Peppa M, He C, Vlassara H. (2002), Mol Med. 8:337-46.
3. Monnier, V., and Cerami, A. (1981) Science 211, 491-493.
4. Ahmed M.U., Thorpe S.R., Baynes J.W (1986) J. Biol. Chem. 261, 4889-4894.
5. Reddy S., Bichler J., Wells-Knecht K.J., Thorpe S.R., Baynes J.W (1995) Biochemistry 34, 10872-10878.
6. Dunn, J. A., Patrick, J. S., Thorpe, S. R., and Baynes, J. W. (1989) Biochemistry 28, 9464-946
7. Ahmed, M. U., Brinkmann Frye, E., Degenhardt, T. P., Thorpe, S. R., and Baynes, J. W. (1997) Biochem. J. 324, 565-570.
8. Sell, D. R., and Monnier, V. M. (1989) J. Biol. Chem. 264, 21597-21602.
9. Onorato, J., Jenkins, A., Thorpe, S., and Baynes, J. (2000) J. Biol. Chem. 275, 21177-21184.
10. Boehm BO, Schilling S, Rosinger S, Lang GE, Lang GK, Kientsch-Engel P, Stahl P (2004) Diabetologia 47, 1376-1379.
Morgan, P. E. et al. (2014). Perturbation of human coronary artery endothelial cell redox state and NADPH generation by methylglyoxal. PLoS One. 9:e86564.
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Product Notes
The OxiSelect Methylglyoxal (MG) (Catalog #AAA168996) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "OxiSelect Methylglyoxal (MG), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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