IL-21 active protein

Recombinant canine IL-21 protein

Cat. Num. AAA201287

• Gene Names: IL21; IL-21
• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: IL-21; Recombinant canine IL-21 protein; Interleukin-21; IL-21 active protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4)
• Concentration: 0.5mg/ml (determined by absorbance at 280nm) (varies by lot)
• Sequence Positions: 18-146aa
• Sequence: MHKSSFQEQD LLLIRMRQLI DIVDQLKNYV NDLDPESLPA PEDVKRHCER SAFSCFQKVQ LKAANTGGNE QIINVLTKQL KRKLPPTNAG RRQKHRPACP SCDSYEKAPP KEFLERLKSL IQKMIHQHLS

• Applicable Applications for IL-21 active protein: SDS-PAGE, Bioactivity
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Canine
• Tag: Non-Tagged
• Bioactivity: The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 range <= 2ng/ml.
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

Biological Activity

(Canine IL-21 stimulates IFN-g secretion of the NK-92 human natural killer cells. The ED50 range )

Related Product Information for IL-21 active protein

IL-21, also known as Interleukin-21, is an important modulator of natural killer (NK) cell function. However, little is known about IL-21 function in canine NK cells because the phenotype of these cells remains undefined. NK cells proliferated rapidly in response to activation by IL-21 for 3 weeks, and IL-21 was able to induce changes in the mRNA expression of NK cell-related receptors and enhance the effector function of NK cells in perforin- and granzyme-B-dependent manners. The duration, frequency and timing of IL-21 stimulation during culture affected the rate of proliferation, patterns of receptor expression, cytokine production, and anti-tumor activity. It has a potential predicating that synergistic interactions of IL-21 with IL-2 and IL-15 play an important role in the proliferation, receptor expression, and effector function of canine NK cells. Recombinant canine IL-21, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for IL-21 active protein

Immunology; Proteins

References

DJ Shin., et al. (2015) Vet Immunol Immunopathol. 15;165(1-2):22-33. Gui G., et al. (2017) Clin Immunol. 183:266-272. Parrish-Novak J., et al. (2000) Nature. 408(6808):57-63.

NCBI and Uniprot Product Information

• NCBI GI #: 50979194
• NCBI GeneID: 442935
• NCBI Accession #: NP_001003347.1
• NCBI GenBank Nucleotide #: NM_001003347.1
• UniProt Accession #: Q6L7I9
• Molecular Weight: 16,776 Da
• NCBI Official Full Name: interleukin-21
• NCBI Official Symbol: IL21
• NCBI Official Synonym Symbols: IL-21
• NCBI Protein Information: interleukin-21
• UniProt Protein Name: Interleukin-21
• UniProt Gene Name: IL21
• UniProt Synonym Gene Names: IL-21
• UniProt Entry Name: IL21_CANFA

Uniprot Description

Function: Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG1 and IgG3 in B-cells. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation