IL-1 alpha/IL-1F1 Active Protein | IL-1alpha/IL-1F1 active protein

Recombinant canine IL-1 alpha/IL-1F1 protein

Cat. Num. AAA201289

• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: IL-1 alpha/IL-1F1; Recombinant canine IL-1 alpha/IL-1F1 protein; interleukin 1 alpha; IL1A; interleukin-1 alpha precursor;BAF; Hematopoietin-1; IL1 alpha; IL1F1hematopoietin-1; LAF; LEM; preinterleukin 1 alpha; pro-interleukin-1-alpha; IL-1alpha/IL-1F1 active protein

• Host: Baculovirus
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 109-265aa
• Sequence: SVAYNFH NNEKYNYIRI IKSQFILNDN LNQSIVRQTG GNYLMTAALQ NLDDAVKFDM GAYTSEDSKL PVTLRISKTR LFVSAQNEDE PVLLKEMPET PKTIRDETNL LFFWERHGSK HYFKSVAQPK LFIATQERKL VHMARGQPSI TDFRLLETQP

• Applicable Applications for IL-1alpha/IL-1F1 active protein: SDS-PAGE, Bioactivity
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Canine
• Tag: His-Tag
• Predicted N-term: Ala
• Bioactivity: Measured in a cell proliferation assay using D10.G4.1 mouse helper T cell. The ED50 range <= 2ng/ml.
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Biological Activity

(Canine IL-1 alpha/IL-1F1 in a cell proliferation assay using D10.G4.1 mouse helper T cell. The ED50 range )

Related Product Information for IL-1alpha/IL-1F1 active protein

IL-1 alpha /IL-1F1, also known as hematopoietin 1 is a cytokine of the interleukin 1 family. IL-1alpha is a unique member in the cytokine family in the sense that the structure of its initially synthesized precursor does not contain a signal peptide fragment. In general, Interleukin 1 is responsible for the production of inflammation, as well as the promotion of fever and sepsis. IL-1 alpha inhibitors are being developed to interrupt those processes and treat diseases. IL-1 alpha is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Although IL-1 production is generally considered to be a consequence of inflammation, recent evidence suggests that IL-1 is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. Recombinant canine IL-1 alpha /IL-1F1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for IL-1alpha/IL-1F1 active protein

Immunology; Proteins

References

Watanabe N., et al, (1994) Cytokine. 6:597-601. Nicklin MJ., et al, (1994) Genomics. 19:382-384. March CJ, Mosley B., et al, (1985). Nature. 315 : 641-647.

NCBI and Uniprot Product Information

• UniProt Accession #: O46612
• Molecular Weight: 19.3kDa (166aa)
• UniProt Protein Name: Interleukin-1 alpha
• UniProt Gene Name: IL1A
• UniProt Synonym Gene Names: IL-1 alpha

Uniprot Description

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells ().