Erythropoietin/EPO Active Protein | EPO active protein

Recombinant rat Erythropoietin/EPO protein

Cat. Num. AAA201313

• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: Erythropoietin/EPO; Recombinant rat Erythropoietin/EPO protein; Erythropoietin; erythropoietin isoform 1 precursor; Epo; EPO active protein

• Host: HEK293
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 1mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 27-192aa
• Sequence: APPRLIC DSRVLERYIL EAKEAENVTM GCAEGPRLSE NITVPDTKVN FYAWKRMKVE EQAVEVWQGL SLLSEAILQA QALQANSSQP PESLQLHIDK AISGLRSLTS LLRVLGAQKE LMSPPDATQA APLRTLTADT FCKLFRVYSN FLRGKLKLYT GEACRRGDR

• Applicable Applications for EPO active protein: SDS-PAGE, Bioactivity
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Rat
• Tag: His-Tag
• Predicted N-term: Asp
• Bioactivity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range <= 2ng/ml.
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Biological Activity

(Rat EPO stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range )

Related Product Information for EPO active protein

EPO, also known as erythropoietin, is a member of t the type I cytokine family and is related to thrombopoietin. It is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules. This protein can be found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. It also has neuroprotective activity against a variety of potential brain injuries and anti-apoptotic functions in several tissue types. Recombinant rat Erythropoietin/EPO Protein, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Categories/Family for EPO active protein

Angiogenesis; Cardiovascular; Proteins

References

Koury, M.J., et al. (2005) Exp. Hematol. 33:1263. Jelkmann W., et al. (2007) Eur J Haematol. 78:183-205.

NCBI and Uniprot Product Information

• NCBI GI #: 8393316
• NCBI GeneID: 24335
• NCBI Accession #: NP_058697.1
• NCBI GenBank Nucleotide #: NM_017001.1
• UniProt Accession #: P29676; P70504
• Molecular Weight: 21,286 Da
• NCBI Official Full Name: erythropoietin
• NCBI Official Synonym Full Names: erythropoietin
• NCBI Official Symbol: Epo
• NCBI Protein Information: erythropoietin
• UniProt Protein Name: Erythropoietin
• Protein Family: Erythropoietin
• UniProt Gene Name: Epo
• UniProt Entry Name: EPO_RAT

NCBI Description

acts as a regulator of erythropoiesis; may facilitate oxygen delivery in response to hypoxia [RGD, Feb 2006]

Uniprot Description

Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.