SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
IL-2 active protein
Recombinant IL-2
Applications
SDS-Page
Purity
>90% by SDS-PAGE
Synonyms
IL-2; Recombinant IL-2; Interleukin-2; Interleukin 2; IL 2; T-cell growth factor; TCGF; Aldesleukin; Involved in regulation of T cell clonal expansion; Lymphokine; OTTHUMP00000164090; POIL2; IL-2 active protein
Host
E Coli
Purity/Purification
>90% by SDS-PAGE
Form/Format
Liquid; In Phosphate-Buffered Saline (pH 7.4) Containing 10% Glycerol.
Concentration
0.25mg/ml (determined by Bradford assay) (varies by lot)
Sequence Positions
21-155aa
Sequence
MAPTSSPAKE TQQHLEQLLL DLQVLLRGID NYKNLKLPMM LTFKFYLPKQ ATELKHLQCL ENELGALQRV LDLTQSKSFH LEDAGNFISN IRVTVVKLKG SENKFECQFD DEPATVVEFL RRWIAICQSI ISTMTQ
Sequence Length
155
Applicable Applications for IL-2 active protein
SDS-PAGE, Bioactivity
Species
Rat
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Tag
Non-Tagged
Biological Activity
Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is less or equal to 0.3 ng/ml.
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Related Product Information for IL-2 active protein
IL-2, also known as T cell growth factor (TCGF), is an immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. Il-2/ Il-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. Recombinant rat IL-2 was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for IL-2 active protein
References
Watson J., et al. (1980). Immunol Rev. 51:257-78. Taniguchi T., et al. (1983). Nature. 302(5906): 305-10.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15.6kDa (136aa), confirmed by MALDI-TOF
NCBI Official Full Name
interleukin-2
NCBI Official Synonym Full Names
interleukin 2
NCBI Official Symbol
Il2
NCBI Protein Information
interleukin-2
UniProt Protein Name
Interleukin-2
UniProt Gene Name
Il2
UniProt Synonym Gene Names
Il-2; IL-2; TCGF
UniProt Entry Name
IL2_RAT
NCBI Description
cytokine produced by T-cells in response to antigen or mitogen stimulation [RGD, Feb 2006]
Uniprot Description
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Research Articles on IL-2
Similar Products
Product Notes
The IL-2 il2 (Catalog #AAA201154) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 21-155aa. AAA Biotech's IL-2 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Bioactivity. Researchers should empirically determine the suitability of the IL-2 il2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MAPTSSPAKE TQQHLEQLLL DLQVLLRGID NYKNLKLPMM LTFKFYLPKQ ATELKHLQCL ENELGALQRV LDLTQSKSFH LEDAGNFISN IRVTVVKLKG SENKFECQFD DEPATVVEFL RRWIAICQSI ISTMTQ. It is sometimes possible for the material contained within the vial of "IL-2, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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