Erythropoietin/EPO Active Protein | EPO active protein

Recombinant Mouse Erythropoietin/EPO Protein

Cat. Num. AAA201272

• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: Erythropoietin/EPO; Recombinant Mouse Erythropoietin/EPO Protein; Erythropoietin; erythropoietin isoform 1 precursor; Epo; EPO active protein

• Host: Baculovirus
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 27-192aa
• Sequence: MAPPRLI CDSRVLERYI LEAKEAENVT MGCAEGPRLS ENITVPDTKV NFYAWKRMEV EEQAIEVWQG LSLLSEAILQ AQALLANSSQ PPETLQLHID KAISGLRSLT SLLRVLGAQK ELMSPPDTTP PAPLRTLTVD TFCKLFRVYA NFLRGKLKLY TGEVCRRGDR

• Applicable Applications for EPO active protein: SDS-PAGE, Bioactivity
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Mouse
• Tag: His-Tag
• Predicted N-term: Ala
• Bioactivity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range <= 2ng/ml.
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Biological Activity

(Mouse EPO stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range )

Related Product Information for EPO active protein

EPO, also known as erythropoietin, is a glycoprotein hormone in the type I cytokine family and is related to thrombopoietin. It is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules. This protein can be found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. It also has neuroprotective activity against a variety of potential brain injuries and anti-apoptotic functions in several tissue types. Recombinant mouse EPO, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for EPO active protein

Angiogenesis; Cardiovascular; Proteins

References

Koury, M.J., et al. (2005) Exp. Hematol. 33:1263. Jelkmann W., et al. (2007) Eur J Haematol. 78:183-205.

NCBI and Uniprot Product Information

• UniProt Accession #: P07321
• Molecular Weight: 19.8kDa (176aa)
• UniProt Protein Name: Erythropoietin
• Protein Family: Erythropoietin
• UniProt Gene Name: Epo
• UniProt Entry Name: EPO_MOUSE

Uniprot Description

EPO: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Genetic variation in EPO is associated with susceptbility to microvascular complications of diabetes type 2 (MVCD2). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the EPO/TPO family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; cell surface; extracellular region

Molecular Function: erythropoietin receptor binding; hormone activity; protein kinase activator activity

Biological Process: hemoglobin biosynthetic process; heart morphogenesis; erythrocyte maturation; apoptosis; positive regulation of transcription, DNA-dependent; regulation of transcription from RNA polymerase II promoter; negative regulation of myeloid cell apoptosis; peptidyl-serine phosphorylation; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of protein kinase activity; positive regulation of cell proliferation; cardiac muscle morphogensis; response to hypoxia; erythrocyte differentiation; ventricular cardiac muscle morphogenesis; angiogenesis; positive regulation of Ras protein signal transduction; positive regulation of neuron differentiation; embryo implantation; positive regulation of DNA replication; negative regulation of apoptosis