Human secretory leukocyte protease inhibitor, SLPI ELISA Kit | SLPI elisa kit

Human secretory leukocyte protease inhibitor, SLPI ELISA Kit

Cat. Num. AAA161395

• Gene Names: SLPI; ALP; MPI; ALK1; BLPI; HUSI; WAP4; WFDC4; HUSI-I
• Reactivity: Human
• Synonyms: secretory leukocyte protease inhibitor; SLPI; Human secretory leukocyte protease inhibitor; SLPI ELISA Kit; SLPI elisa kit

• Reactivity: Human
• Sequence Length: 132

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 20ng/L - 4500ng/L
• Sensitivity: 10.23ng/L
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SLPI elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Secretory Leukocyte Protease Inhibitor (also known as SLPI) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human SLPI antibody. SLPI present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human SLPI Antibody is added and binds to SLPI in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SLPI antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human SLPI. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 758101
• NCBI GeneID: 6590
• UniProt Accession #: P03973; P07757; B2R5H8
• Molecular Weight: 14,326 Da
• NCBI Official Full Name: secretory leukocyte protease inhibitor (SLPI)
• NCBI Official Synonym Full Names: secretory leukocyte peptidase inhibitor
• NCBI Official Symbol: SLPI
• NCBI Official Synonym Symbols: ALP; MPI; ALK1; BLPI; HUSI; WAP4; WFDC4; HUSI-I
• NCBI Protein Information: antileukoproteinase
• UniProt Protein Name: Antileukoproteinase
• Protein Family: Antileukoproteinase
• UniProt Gene Name: SLPI
• UniProt Synonym Gene Names: WAP4; WFDC4; MPI

NCBI Description

This gene encodes a secreted inhibitor which protects epithelial tissues from serine proteases. It is found in various secretions including seminal plasma, cervical mucus, and bronchial secretions, and has affinity for trypsin, leukocyte elastase, and cathepsin G. Its inhibitory effect contributes to the immune response by protecting epithelial surfaces from attack by endogenous proteolytic enzymes. This antimicrobial protein has antibacterial, antifungal and antiviral activity. [provided by RefSeq, Nov 2014]

Uniprot Description

Acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G (PubMed:3533531, PubMed:3462719, PubMed:2039600, PubMed:2110563, PubMed:10702419, PubMed:24121345). Modulates the inflammatory and immune responses after bacterial infection, and after infection by the intracellular parasite L.major. Down-regulates responses to bacterial lipopolysaccharide (LPS) (). Plays a role in regulating the activation of NF-kappa-B and inflammatory responses (PubMed:10702419, PubMed:24352879). Has antimicrobial activity against mycobacteria, but not against salmonella. Contributes to normal resistance against infection by M.tuberculosis. Required for normal resistance to infection by L.major. Required for normal wound healing, probably by preventing tissue damage by limiting protease activity (). Together with ELANE, required for normal differentiation and proliferation of bone marrow myeloid cells (PubMed:24352879).