IL-21 active protein

Recombinant Mouse IL-21 Protein

Cat. Num. AAA201362

• Gene Names: Il21; IL-21
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: IL-21; Recombinant Mouse IL-21 Protein; Interleukin-21; CVID11; IL21; IL-21Za11interleukin-21; Interleukin-21 isoform; Za11; IL-21 active protein

• Host: E Coli
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4)
• Concentration: 1mg/ml (determined by Bradford assay) (varies by lot)
• Sequence Positions: 18-146aa
• Sequence: MHKSSPQGPD RLLIRLRHLI DIVEQLKIYE NDLDPELLSA PQDVKGHCEH AAFACFQKAK LKPSNPGNNK TFIIDLVAQL RRRLPARRGG KKQKHIAKCP SCDSYEKRTP KEFLERLKWL LQKMIHQHLS

• Applicable Applications for IL-21 active protein: SDS-PAGE, Bioactivity
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Mouse
• Tag: Non-Tagged
• Bioactivity: The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 range <= 100ng/ml.
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

Biological Activity

(Mouse IL-21 stimulates IFN-g secretion of the NK-92 human natural killer cells. The ED50 range )

Related Product Information for IL-21 active protein

IL-21, also known as Interleukin-21, is produced from activated helper T cells such as Th17 cells and is a member of the common-gamma chain family of cytokines that includes IL-2, IL-4, IL-7, IL-9, and IL-15. Previous studies have shown that IL-21 plays an important role in the regulation of both innate and adaptive immune responses. IL-21 is critical for NK cell activation and expansion. IL-21 also promotes terminal B cell differentiation into plasma cells and antibody production. Furthermore, IL-21 enhances antigen-specific CD8 T cell expansion and the differentiation of follicular helper T cells in vivo. IL-21 also promotes the differentiation of the inflammatory Th17 lineage and contributes to autoimmunity. IL-21 isoform is an alternative splice variant form of IL-21 that we previously identified and reported to have a similar function as original IL-21. Follicular helper T cells also produce IL-21 to activate and differentiate B cells. Recombinant mouse IL-21, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for IL-21 active protein

Hormones & Cytokines; Proteins

References

Yuqing Yuan., et al. (2017) JCI Insight. 6;2(7):e90652. Akemi Araki., et al. (2019) J Immunol. 202:3326-3333.

NCBI and Uniprot Product Information

• NCBI GI #: 11140821
• NCBI GeneID: 60505
• NCBI Accession #: NP_068554.1
• NCBI GenBank Nucleotide #: NM_021782.3
• UniProt Accession #: Q9ES17
• Molecular Weight: 16,811 Da
• NCBI Official Full Name: interleukin-21 isoform 2
• NCBI Official Synonym Full Names: interleukin 21
• NCBI Official Symbol: Il21
• NCBI Official Synonym Symbols: IL-21
• NCBI Protein Information: interleukin-21
• UniProt Protein Name: Interleukin-21
• UniProt Gene Name: Il21
• UniProt Synonym Gene Names: IL-21
• UniProt Entry Name: IL21_MOUSE

Uniprot Description

IL21: Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. Belongs to the IL-15/IL-21 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Cytokine; Secreted; Cell development/differentiation; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interleukin-2 receptor binding; cytokine activity

Biological Process: positive regulation of interleukin-17 production; positive regulation of natural killer cell cytokine production; cell maturation; B cell apoptosis; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of interleukin-10 production; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of natural killer cell differentiation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of cell proliferation; positive regulation of B cell proliferation; positive regulation of B cell differentiation; positive regulation of T cell proliferation; immune response; positive regulation of interferon-gamma biosynthetic process