Rat E-Cadherin PicoKine ELISA Kit | Cdh1 elisa kit

Rat E-Cadherin PicoKine ELISA Kit

Cat. Num. AAA177628

• Reactivity: Rat
• Synonyms: E-Cadherin PicoKine; Rat E-Cadherin PicoKine ELISA Kit; Cadherin-1; Arc 1; Cadherin 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD 234; CD324; CD324 antigen; CDH1; CDHE; ECAD; Epithelial cadherin; epithelial calcium dependant adhesion protein; LCAM; Liver cell adhesion molecule; UVO; Uvomorulin; cadherin 1; type 1; E-cadherin (epithelial); Cdh1 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant rat E-Cadherin
• Sequence Length: 886

• Assay Range: 156pg/ml-10000pg/ml
• Sensitivity: <15pg/ml
• Sample: For quantitative detection of rat E-Cadherin in cell culture supernates, serum and plasma(heparin).
• Immunogen: NSO, Q24-S704
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml rat E-Cadherin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each rat E-Cadherin standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|1571|4158|7193|1690|4873|8056; Standard deviation|75.41|228.69|431.58|119.99|302.13|459.19; CV(%)|4.8|5.5|6.0|7.1|6.2|5.7
• Sample Data: Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000; O.D.|0.097|0.100|0.163|0.252|0.482|0.930|1.654|2.438
• Anticoagulant: Heparin
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Rat E-Cadherin PicoKine ELISA Kit standard curve )

Related Product Information for Cdh1 elisa kit

Principle of the assay: Rat E-Cadherin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for E-Cadherin has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: Q24-S704) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for E-Cadherin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat E-Cadherin amount of sample captured in plate.

Background: Cadherin-1, also known as CAM 120/80 or epithelial cadherin (E-cadherin) or uvomorulin, is a protein that in humans is encoded by the CDH1 gene. E-cadherin is a Ca(2+)-dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. The gene spans a region of approximately 100 kb, and its location on chromosome 16q22.1. It contains 16 exons and a 65-kb-long intron 2. E-cadherin gene mutations may contribute to the development of diffusely growing gastric carcinomas. It plays a central part in the process of epithelial morphogenesis and acts as a strong invasion suppressor in experimental tumor cell systems. The standard product used in this kit is recombinant gene expression with the molecular mass of 120KDa.

NCBI and Uniprot Product Information

• NCBI GI #: 13786164
• NCBI GeneID: 83502
• NCBI Accession #: NP_112624.1
• NCBI GenBank Nucleotide #: NM_031334.1
• UniProt Accession #: Q9R0T4; O35794; Q9JIV9
• Molecular Weight: 98,715 Da
• NCBI Official Full Name: cadherin-1
• NCBI Official Synonym Full Names: cadherin 1
• NCBI Official Symbol: Cdh1
• NCBI Protein Information: cadherin-1
• UniProt Protein Name: Cadherin-1
• Protein Family: Cadherin
• UniProt Gene Name: Cdh1
• UniProt Synonym Gene Names: E-cadherin
• UniProt Entry Name: CADH1_RAT

NCBI Description

cell-cell adhesion molecule; may play a role in axonal growth and synase formation [RGD, Feb 2006]

Uniprot Description

CDH1: a single-pass type I membrane protein, and calcium dependent cell adhesion proteins. It is a ligand for integrin alpha-E/beta-7, and it colocalizes with DLG7 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Defects in CDH1 are involved in dysfunction of the cell-cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis. Has a potent invasive suppressor role.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Ubiquitin ligase; Membrane protein, integral

Cellular Component: actin cytoskeleton; adherens junction; apical junction complex; apical part of cell; axon; basolateral plasma membrane; catenin complex; cell junction; cell surface; cell-cell adherens junction; cortical actin cytoskeleton; cytoplasm; endosome; flotillin complex; focal adhesion; integral to membrane; intercellular junction; internal side of plasma membrane; lamellipodium; lateral loop; lateral plasma membrane; nerve terminal; perinuclear region of cytoplasm; plasma membrane; trans-Golgi network

Molecular Function: ankyrin binding; beta-catenin binding; calcium ion binding; cell adhesion molecule binding; gamma-catenin binding; glycoprotein binding; GTPase activating protein binding; protein binding; protein domain specific binding; protein phosphatase binding

Biological Process: calcium-dependent cell-cell adhesion; cell-cell adhesion; decidualization; embryo implantation; homophilic cell adhesion; in utero embryonic development; negative regulation of cell-cell adhesion; negative regulation of epithelial cell proliferation; neurite development; pituitary gland development; positive regulation of transcription factor import into nucleus; positive regulation of transcription, DNA-dependent; protein homooligomerization; protein metabolic process; regulation of caspase activity; regulation of protein localization; regulation of water loss via skin; response to drug; response to organic substance; response to toxin; sensory perception of sound; synaptogenesis; trophectodermal cell differentiation