Rabbit anti-Human Cleaved-Endostatin Polyclonal Antibody | anti-H1572 antibody

Cleaved-Endostatin (H1572) polyclonal antibody

Cat. Num. AAA3013917

• Gene Names: COL18A1; KS; KNO; GLCC; KNO1
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Cleaved-Endostatin; Polyclonal Antibody; Cleaved-Endostatin (H1572) polyclonal antibody; Collagen alpha-1(XVIII) chain; Endostatin; Non-collagenous domain 1; NC1; COL18A1; anti-H1572 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Cleaved-Endostatin (H1572) polyclonal antibody detects endogenous levels of Cleaved-Endostatin protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1516

• Applicable Applications for anti-H1572 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 1572-1754 of Human Cleaved-Endostatin.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Cleaved-Endostatin (H1572)  polyclonal antibody at 1:500 dilutionLane1:MCF-7 whole cell lysate (40ug) Lane2:L02 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug))

Related Product Information for anti-H1572 antibody

Collagen type XV (COL15 gene product) and XVIII (COL18 gene product) form the new subgroup MULTIPLEXIN, within the diverse family of collagens, which contains 19 distinct types of collagens found in vertebrates. Both Collagen Type XV and Type XVIII are characterized by extensive interruptions in their collagenous sequences. Members of the MULTIPLEXIN subgroup contain polypeptides with multiple triple-helical domains separated and flanked by nontriple-helical regions. Type XV is predominantly expressed in internal organs such as adrenal gland, kidney and pancreas. Type XVIII encodes 2 different alpha1 chains, which have different signal peptides and variant N-terminal non-collagenous NC1 domains of 495 and 303 amino acids. The long variant NC1-434 Type XVIII mRNAs are prominently expressed in liver, while the variant NC1- 303 mRNAs are predominantly expressed in heart, kidney, placenta, prostate, ovary, skeletal muscle and small intestine. Endostatin is a fragment of the Cterminal domain NC1 of Collagen Type XV and Type XVIII that inhibits angiogenesis and tumor growth. Unlike endostatin-XVIII, endostatin-XV does not bind zinc or heparin, however both endostatins can inhibit chorioallantoic membrane angiogenesis induced by basic FGF or VEGF. Collagen Type XV and XVIII are also widely present in basement membrane zones, suggesting their roles in basement membrane-stromal interactions and in-volvement with angiogenic and pathological processes.

NCBI and Uniprot Product Information

• NCBI GI #: 110611235
• NCBI GeneID: 80781
• NCBI Accession #: NP_085059.2
• NCBI GenBank Nucleotide #: NP_085059.2
• UniProt Accession #: P39060
• Molecular Weight: ~ 20kDa
• NCBI Official Full Name: collagen alpha-1(XVIII) chain isoform 1 preproprotein
• NCBI Official Synonym Full Names: collagen type XVIII alpha 1 chain
• NCBI Official Symbol: COL18A1
• NCBI Official Synonym Symbols: KS; KNO; GLCC; KNO1
• NCBI Protein Information: collagen alpha-1(XVIII) chain
• UniProt Protein Name: Collagen alpha-1(XVIII) chain
• UniProt Gene Name: COL18A1
• UniProt Entry Name: COIA1_HUMAN

NCBI Description

This gene encodes the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. A long isoform of the protein has an N-terminal domain that is homologous to the extracellular part of frizzled receptors. Proteolytic processing at several endogenous cleavage sites in the C-terminal domain results in production of endostatin, a potent antiangiogenic protein that is able to inhibit angiogenesis and tumor growth. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

COL18A1: COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube. Defects in COL18A1 are a cause of Knobloch syndrome type 1 (KNO1). An autosomal recessive disorder defined by the occurrence of high myopia, vitreoretinal degeneration with retinal detachment, macular abnormalities and occipital encephalocele. Belongs to the multiplexin collagen family. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: extracellular matrix; extracellular space; collagen; endoplasmic reticulum lumen; extracellular region; basement membrane

Molecular Function: identical protein binding; protein binding; metal ion binding; structural molecule activity

Biological Process: response to drug; extracellular matrix organization and biogenesis; collagen catabolic process; extracellular matrix disassembly; negative regulation of cell proliferation; organ morphogenesis; visual perception; positive regulation of cell proliferation; endothelial cell morphogenesis; angiogenesis; cell adhesion; response to hydrostatic pressure; positive regulation of cell migration

Disease: Knobloch Syndrome 1